FastQCFastQC Report
Wed 25 May 2016
SRR1781285_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781285_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences178356
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT82354.617170154073875No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT68853.860257014061764No Hit
CAAATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT58273.267061382852273No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG46992.6346184036421536No Hit
CATATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT31081.7425822512278815No Hit
TCATATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG24911.3966449124223463No Hit
TCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG23281.305254659220884No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC14010.7855076364125682No Hit
CTAATACCCATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT5800.32519231200520304No Hit
CACTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC5740.3218282536051493No Hit
CAAATACCCATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT5690.3190248716051044No Hit
CAAAAACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT4860.27248873040435984No Hit
CTAAAACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT3800.21305703200340892No Hit
TCTTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC3720.20857162080333713No Hit
TCATTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC3320.1861445648029783No Hit
AAAATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT2730.15306465720244905No Hit
CTAATACACATCTCCGCGCCCACGAGACATAAGTTAATCTCGTATGCCGT2590.14521518760232344No Hit
CTTATACCCATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT2530.1418511292022696No Hit
CAAATACACATCTCCGCGCCCACGAGACATAAGTTAATCTCGTATGCCGT2270.12727354280203637No Hit
CTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTC2150.12054542600192872No Hit
CATATACCCATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT2120.11886339680190182No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGATAATCTCGTATGCCGT2040.11437798560183005No Hit
ATAATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT1960.10989257440175827No Hit
CAAATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGACGT1880.10540716320168653No Hit
CTAATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGACGT1820.10204310480163269No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACTAA207.882851E-570.049148
ACTAATG207.8938174E-570.0294650
GCTTAAA207.904795E-570.0098256
ACGTAAG207.904795E-570.0098240
CAAGTAT150.002220579770.0098139
CACGTAA150.002220579770.0098139
TTAATAC207.9157864E-569.990193
ACCACAT252.8335162E-669.990195
GATTATC150.002223051269.9901933
CAAATAC11700.064.606321
TAATACC1250.064.390972
AGGGGGG25400.064.203670
TCTTTAT550.063.6274451
AAATACA11950.062.9618842
CTAATAC15800.062.6810841
AATACAC27350.061.9291573
TCGTATC401.0591975E-861.2585940
TAATACA15700.060.6284452
CGTATCC352.9530202E-760.02524641
GATAGAA352.95875E-760.00841556
Produced by FastQC (version 0.11.5)