FastQCFastQC Report
Wed 25 May 2016
SRR1781284_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781284_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences153452
Sequences flagged as poor quality0
Sequence length76
%GC72

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4288427.946198159685114No Hit
CTTATACACATCTGACGCTGCCGACGAGGGCCGGTGTGTAGATCTCGGTG20981.3672027735057217No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5450.35515991971430805No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2850.18572582957537212No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2340.15249068112504235No Hit
TCTTATACACATCTGACGCTGCCGACGAGGGCCGGTGTGTAGATCTCGGT1970.12837890675911687No Hit
GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1650.10752548028047859No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAAGA150.00221863970.020538
AGAACCG150.002221509669.9977212
TTGTGCG252.3848751E-455.9981772
GTTAGTG200.006932569752.5154047
CCAGTTG200.006932569752.5154049
AACGTGA200.00694151452.4982946
CTGACAT200.00694151452.4982943
GTAGATC3550.049.2941738
GTATCAT3500.048.99840557
TCTCGGT3650.048.90251543
GCCGTAT3400.048.38077554
AGATCTC3700.048.24167340
CGCCGTA3500.047.9984453
ATCATTA3500.047.9984459
TAGATCT3650.047.9436439
TGGTCGC3650.047.9436449
CCGTATC3450.047.67960755
CATTAAA3600.047.63733361
TATCATT3550.047.32240358
CGTATCA3550.047.32240356
Produced by FastQC (version 0.11.5)