##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1781284_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 153452 Sequences flagged as poor quality 0 Sequence length 76 %GC 72 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.763398326512526 32.0 27.0 32.0 13.0 32.0 2 28.4268500899304 32.0 27.0 32.0 13.0 32.0 3 28.097437635221436 32.0 27.0 32.0 13.0 32.0 4 27.23667987383677 32.0 27.0 32.0 13.0 32.0 5 28.074590099835778 32.0 27.0 32.0 13.0 32.0 6 31.89928446680395 37.0 32.0 37.0 13.0 37.0 7 31.4785731042932 37.0 32.0 37.0 13.0 37.0 8 31.955745118994866 37.0 32.0 37.0 13.0 37.0 9 31.942607460313322 37.0 32.0 37.0 13.0 37.0 10-11 31.55697872950499 37.0 32.0 37.0 13.0 37.0 12-13 31.38735891353648 37.0 29.5 37.0 13.0 37.0 14-15 32.35005734692282 37.0 32.0 37.0 13.0 37.0 16-17 32.490938534525455 37.0 34.5 37.0 13.0 37.0 18-19 31.73786591246774 37.0 29.5 37.0 13.0 37.0 20-21 32.30119842035295 37.0 32.0 37.0 13.0 37.0 22-23 32.28241404478273 37.0 32.0 37.0 13.0 37.0 24-25 31.724539269608737 37.0 32.0 37.0 13.0 37.0 26-27 32.313928785548576 37.0 32.0 37.0 13.0 37.0 28-29 31.758927873211167 37.0 32.0 37.0 13.0 37.0 30-31 31.98465969814665 37.0 32.0 37.0 13.0 37.0 32-33 31.944467976956965 37.0 32.0 37.0 13.0 37.0 34-35 31.954334254359672 37.0 32.0 37.0 13.0 37.0 36-37 32.70477087297657 37.0 32.0 37.0 13.0 37.0 38-39 32.55734366446837 37.0 32.0 37.0 13.0 37.0 40-41 31.83012929124417 37.0 32.0 37.0 13.0 37.0 42-43 31.90144800980111 37.0 32.0 37.0 13.0 37.0 44-45 31.58848695357506 37.0 32.0 37.0 13.0 37.0 46-47 31.94130086281052 37.0 32.0 37.0 13.0 37.0 48-49 32.499309230247896 37.0 32.0 37.0 13.0 37.0 50-51 32.74345723744233 37.0 32.0 37.0 13.0 37.0 52-53 31.73415791257201 37.0 32.0 37.0 13.0 37.0 54-55 31.911135078070014 37.0 32.0 37.0 13.0 37.0 56-57 31.645833876391315 37.0 29.5 37.0 13.0 37.0 58-59 31.164230508562937 37.0 29.5 37.0 13.0 37.0 60-61 30.931232567838805 37.0 27.0 37.0 13.0 37.0 62-63 30.545577770247373 37.0 27.0 37.0 10.5 37.0 64-65 30.381914214216824 37.0 27.0 37.0 10.5 37.0 66-67 30.457690352683574 37.0 27.0 37.0 13.0 37.0 68-69 30.763323384511118 37.0 27.0 37.0 13.0 37.0 70-71 30.857724239501604 37.0 27.0 37.0 13.0 37.0 72-73 31.279478273336288 37.0 29.5 37.0 13.0 37.0 74-75 31.59347874253838 37.0 32.0 37.0 13.0 37.0 76 31.782629095743296 37.0 32.0 37.0 13.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 11101 1 0.6983876853617552 11101 2 1.3522172949317763 11101 3 0.08217748083758991 11101 4 1.1828787157813778 11101 5 0.7897995920194312 11101 6 1.3002714162421967 11101 7 0.3898941515216059 11101 8 1.412079523274791 11101 9 1.6718534725707528 11101 10-11 -0.04409782820757968 11101 12-13 1.1758218136634788 11101 14-15 -0.23851716316125504 11101 16-17 0.2718058058695476 11101 18-19 0.8806022930670849 11101 20-21 0.49490048872849 11101 22-23 0.05096446268292709 11101 24-25 0.6950030135375158 11101 26-27 0.9427109918407055 11101 28-29 -0.6489518144890134 11101 30-31 0.7023037406188521 11101 32-33 0.3524359174556011 11101 34-35 0.0920581222677157 11101 36-37 -1.637187774177022 11101 38-39 1.3832914355158508 11101 40-41 -0.7117524235279191 11101 42-43 -0.6974497763169509 11101 44-45 0.733634583250975 11101 46-47 0.76682188518215 11101 48-49 -1.1258244460459679 11101 50-51 0.11440875145882501 11101 52-53 0.7042858770955895 11101 54-55 1.106293443954101 11101 56-57 1.2132184349368877 11101 58-59 -0.48068904621140973 11101 60-61 0.38037283668891675 11101 62-63 1.3090556481144802 11101 64-65 -0.026567787042026225 11101 66-67 0.5043288292941135 11101 68-69 -0.10140386819407965 11101 70-71 0.02127122918581037 11101 72-73 -0.09078139689688669 11101 74-75 -0.37853848707857196 11101 76 -0.8711683186756787 11102 1 -1.2317835926356118 11102 2 0.3930605096880342 11102 3 -0.04101092495951164 11102 4 -0.3731160141263956 11102 5 -0.365668128665682 11102 6 0.1000079116308683 11102 7 -0.41708872067865954 11102 8 0.5240689830903378 11102 9 -0.5817697158350441 11102 10-11 -0.13237187300336117 11102 12-13 0.597099811028432 11102 14-15 0.7054881069309715 11102 16-17 0.04486245936099209 11102 18-19 -0.24489177807916107 11102 20-21 -0.27683864170629136 11102 22-23 0.17777605688582554 11102 24-25 0.9578488633398905 11102 26-27 1.103119423988268 11102 28-29 -0.3175947657406617 11102 30-31 1.6868228447031726 11102 32-33 1.3089576565860312 11102 34-35 0.6085271604758873 11102 36-37 0.542324742292017 11102 38-39 0.6013415013920067 11102 40-41 0.8985901324668077 11102 42-43 0.6171088534590723 11102 44-45 0.7807360325263417 11102 46-47 -0.05004240994302123 11102 48-49 0.44993312971160293 11102 50-51 1.258018764634052 11102 52-53 0.20922658855803533 11102 54-55 -0.08211235314734822 11102 56-57 0.06763213717667682 11102 58-59 -0.5916903637344681 11102 60-61 0.6613346285202724 11102 62-63 -0.2288231397643088 11102 64-65 0.2909552696114588 11102 66-67 -0.15772650667426547 11102 68-69 -0.5177411540965835 11102 70-71 0.02160060994997437 11102 72-73 0.9349103027078556 11102 74-75 -0.32254375717079853 11102 76 0.20129544944026279 11103 1 0.3027808013037827 11103 2 -1.0619584297059035 11103 3 0.1673224083738205 11103 4 0.4512211070857255 11103 5 0.3142561137585602 11103 6 1.070651851024806 11103 7 -1.132524326739265 11103 8 -1.7860635926672401 11103 9 -0.7067697158350441 11103 10-11 -1.35988134270033 11103 12-13 -0.38656496169884136 11103 14-15 0.5740960614764248 11103 16-17 -0.8968989042753748 11103 18-19 -1.1710281417155244 11103 20-21 -0.11798447503962706 11103 22-23 -1.7232656097808388 11103 24-25 -2.5355223487813277 11103 26-27 -0.29460784873900536 11103 28-29 -0.23307772028611495 11103 30-31 0.6366334507637816 11103 32-33 -0.06343817674729735 11103 34-35 -0.19473988497865946 11103 36-37 0.028120196837470246 11103 38-39 0.13827331957381972 11103 40-41 -0.4231428220786455 11103 42-43 -1.1307604647227478 11103 44-45 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11105 64-65 -0.44000296134677086 11105 66-67 -2.173697022644781 11105 68-69 -0.4307223170777448 11105 70-71 0.018529356878719483 11105 72-73 -1.1064492386516847 11105 74-75 -0.5631252477522892 11105 76 -0.06897482083000739 11106 1 -0.8092727701247888 11106 2 -0.0034762868487590026 11106 3 -0.8791061630547503 11106 4 0.30523896422858243 11106 5 0.23389897090141787 11106 6 -1.403455291832337 11106 7 -0.15172075531069495 11106 8 0.5483114073327613 11106 9 1.1468017127363836 11106 10-11 -0.016354556986048152 11106 12-13 -1.5327703188416955 11106 14-15 0.20289070433356926 11106 16-17 -0.840202475703947 11106 18-19 -0.3228138560012397 11106 20-21 -1.638743403611059 11106 22-23 -0.038890609780835916 11106 24-25 0.6885847940758154 11106 26-27 -0.0481792773104317 11106 28-29 0.37071692257103095 11106 30-31 -1.17854512066479 11106 32-33 -0.21008996246158063 11106 34-35 -0.19407024212151924 11106 36-37 -0.9531298031625326 11106 38-39 -1.3876195375690372 11106 40-41 -0.8559553220786462 11106 42-43 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0.061905947038290776 11107 22-23 -0.8103191812094117 11107 24-25 -0.3127139072228857 11107 26-27 0.5674051382739833 11107 28-29 0.9616260134801173 11107 30-31 -0.43114252326219216 11107 32-33 -0.381518533890155 11107 34-35 0.2708648228135502 11107 36-37 -0.11027266030539096 11107 38-39 0.5364064364569359 11107 40-41 0.304434288310965 11107 42-43 0.1852906716408924 11107 44-45 -0.10346310167279782 11107 46-47 0.03870217880157156 11107 48-49 1.151231831010307 11107 50-51 0.11949062610591454 11107 52-53 -0.3481327188012706 11107 54-55 0.032606261571267225 11107 56-57 -0.5882120186674804 11107 58-59 1.0089589869148838 11107 60-61 0.8377415549272023 11107 62-63 -0.374927035868204 11107 64-65 0.00524098389717409 11107 66-67 0.12798777904001923 11107 68-69 0.7636441272886998 11107 70-71 1.0053668437162067 11107 72-73 -1.0434446756471232 11107 74-75 -0.30522773985478224 11107 76 -0.4090941609493477 11108 1 -1.2448209428822636 11108 2 -0.9649380808686949 11108 3 0.19929915255986685 11108 4 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62-63 0.0920013845485883 11108 64-65 0.22435907510828912 11108 66-67 -1.122314244517817 11108 68-69 0.6723575069323111 11108 70-71 -0.13326196933121182 11108 72-73 0.36919501024837587 11108 74-75 -0.13949231249144844 11108 76 -1.066146411024853 11109 1 0.1978700870180674 11109 2 0.429857046484571 11109 3 0.6970843131357256 11109 4 0.4980961070857255 11109 5 0.13866087566332297 11109 6 0.7179732795962366 11109 7 -0.49933980292973956 11109 8 0.6506923597137089 11109 9 -0.15319828726361706 11109 10-11 0.3741216334901445 11109 12-13 -0.5982465093178888 11109 14-15 0.17789070433356358 11109 16-17 1.2883689528674864 11109 18-19 -0.17995671314409734 11109 20-21 0.5862565963889423 11109 22-23 0.7849189140286867 11109 24-25 -0.12927234878132765 11109 26-27 -0.08627451540567677 11109 28-29 0.031431208285312096 11109 30-31 1.1559786888590224 11109 32-33 0.16014813277651285 11109 34-35 0.539263091211815 11109 36-37 -0.14003456506728895 11109 38-39 -0.5804766804261803 11109 40-41 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0.10416381342478331 21610 74-75 0.8367319264951263 21610 76 1.4370678071638388 21611 1 -0.14931960853228432 21611 2 -0.013546544460002252 21611 3 -0.7322677555606063 21611 4 -1.3830514338978794 21611 5 0.04069053998806993 21611 6 -1.3376473293030635 21611 7 -0.47396899887041144 21611 8 -1.7893935107000267 21611 9 -0.4506221748514392 21611 10-11 -1.237314744339674 21611 12-13 0.7055200792847636 21611 14-15 0.08216470901740536 21611 16-17 -0.10565915017701855 21611 18-19 -0.7273806007319195 21611 20-21 -0.19213918815438547 21611 22-23 0.06848643939949284 21611 24-25 -0.4346002176337862 21611 26-27 -0.9769848979193299 21611 28-29 0.48195814036962403 21611 30-31 -0.6005591721870367 21611 32-33 -1.315359283304673 21611 34-35 -0.32627164727374236 21611 36-37 0.13211609847681416 21611 38-39 0.09985118842627827 21611 40-41 -0.8428405679802857 21611 42-43 -0.03713853849324167 21611 44-45 0.10177428389245691 21611 46-47 -1.3550597969276161 21611 48-49 0.8195307452108622 21611 50-51 -0.16659623784980226 21611 52-53 0.20487984240801538 21611 54-55 -0.13675716189051457 21611 56-57 -0.3898939433002262 21611 58-59 0.06106671525212093 21611 60-61 -1.2543384961692468 21611 62-63 -0.07755637374344815 21611 64-65 -0.1650804978997158 21611 66-67 -1.1517652548113766 21611 68-69 -0.19744805921332542 21611 70-71 -0.6553740547296094 21611 72-73 0.48471159431242583 21611 74-75 0.705151225442215 21611 76 0.3406397117353457 21612 1 -0.5733813887788628 21612 2 -0.84385409086293 21612 3 -0.3068511453451883 21612 4 0.5828068508873798 21612 5 -0.28853314243978545 21612 6 0.5022117683801781 21612 7 -0.7710832110367853 21612 8 0.8904601676633419 21612 9 -0.23879450922347445 21612 10-11 0.5673526449029715 21612 12-13 -1.508272089798016 21612 14-15 0.13937240445162757 21612 16-17 -0.07703836708529366 21612 18-19 0.31915780869769605 21612 20-21 -0.22380833867599392 21612 22-23 -0.8463286263097665 21612 24-25 -0.526999621508601 21612 26-27 0.49774752316182 21612 28-29 -1.5696608814431379 21612 30-31 -0.34554630130233477 21612 32-33 -0.43346658583820386 21612 34-35 0.030014763781672116 21612 36-37 -0.009328150269965363 21612 38-39 -0.15072461431047657 21612 40-41 0.45437525643374954 21612 42-43 0.04892703527725217 21612 44-45 0.27729250635553626 21612 46-47 -0.15197078459866376 21612 48-49 0.5160488321909469 21612 50-51 0.8007184891519614 21612 52-53 0.9440750734065197 21612 54-55 0.6341410903788258 21612 56-57 0.261847013209735 21612 58-59 0.8649212065134648 21612 60-61 -0.2195193659700827 21612 62-63 1.1203504139546965 21612 64-65 0.8157486580412154 21612 66-67 -0.6742554322941032 21612 68-69 0.6916252370879903 21612 70-71 1.0112700314375722 21612 72-73 -0.004483636686082093 21612 74-75 1.0466022483388429 21612 76 1.597989664316298 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 7.0 12 29.0 13 84.0 14 211.0 15 333.0 16 562.0 17 907.0 18 1209.0 19 1503.0 20 1904.0 21 2351.0 22 2843.0 23 3504.0 24 3979.0 25 4416.0 26 4844.0 27 5260.0 28 5798.0 29 6462.0 30 7634.0 31 8979.0 32 11080.0 33 14867.0 34 20163.0 35 24041.0 36 20482.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 62.02851706070954 10.581810598754007 5.799859239371269 21.589813101165184 2 50.331048145348376 13.185882230273963 19.591794176680654 16.891275447696998 3 48.975575424236894 13.119411933373302 18.72311862992988 19.18189401245992 4 48.36332119047774 12.674404207206209 15.6564636268255 23.305810975490548 5 48.36887104762401 18.130099314443605 20.288428954982667 13.212600682949718 6 58.87117795792821 19.656309464848942 9.056252117926126 12.41626045929672 7 55.78241488973463 15.89724206245764 9.672983681768416 18.64735936603931 8 54.91133977621521 20.169304859531707 10.282761272327974 14.63659409192511 9 50.93710085238381 10.514688632275892 9.533925918202435 29.01428459713787 10-11 55.559248361860256 14.797183474909009 13.435515456023564 16.208052707207163 12-13 53.708651565310326 12.984190496050882 13.813440033365481 19.49371790527331 14-15 55.148189661913825 12.858092432812867 10.979980710580508 21.0137371946928 16-17 55.43068842374163 13.840158486041238 10.886466126215364 19.842686964001775 18-19 53.678523833263284 13.768193859297565 12.803393906217925 19.749888401221234 20-21 55.37431900529156 13.470661835622867 11.79293850845867 19.362080650626908 22-23 55.42322956513806 13.27166326710155 11.130588917634945 20.17451825012545 24-25 54.56479734639284 14.397057050599049 10.915500988911806 20.12264461409631 26-27 55.70145713317519 14.142859004770223 11.827802830852645 18.32788103120194 28-29 56.807340406120474 13.693858665902042 12.101178218596043 17.397622709381437 30-31 56.045395450679855 12.959795114417258 11.924614617647922 19.07019481725496 32-33 55.19333470184391 13.49937928270496 12.161497280899829 19.14578873455131 34-35 55.75018817020362 13.301922757353301 13.175498447392172 17.772390625050914 36-37 55.58679969501665 12.59652918521222 13.916820353076877 17.899850766694254 38-39 55.01282164071384 12.839887521464435 14.74407223129132 17.4032186065304 40-41 55.08320218440355 13.933392635458109 13.538153748904378 17.445251431233974 42-43 53.77056017516878 13.766193988999817 14.529950733779945 17.933295102051456 44-45 52.800396216394105 13.123364711325728 15.523471585484664 18.552767486795503 46-47 53.96641273109984 13.257326442968765 14.015548937445828 18.760711888485577 48-49 53.77512186221098 13.212274848160988 15.36767197560149 17.644931314026536 50-51 54.734053645439616 13.331530380835702 14.497041420118343 17.43737455360634 52-53 52.08338763913145 14.163386596460132 15.282954930532021 18.47027083387639 54-55 53.1140030758804 13.614354977452233 13.83657430336522 19.43506764330214 56-57 52.90318796757292 14.18130750984021 13.564176419988009 19.35132810259886 58-59 52.42518833250789 15.099184109689023 14.879897296874592 17.595730260928498 60-61 52.03386075124469 14.102781325756588 14.616296952793057 19.247060970205666 62-63 52.37308569566635 15.079830563701533 14.569240795047248 17.977842945584882 64-65 52.42259744152856 15.265459332295 14.759434607790109 17.552508618386327 66-67 52.713072208482814 16.54985451429279 12.606914888417514 18.130158388806887 68-69 52.912157913086546 16.529652691567044 12.462569606683545 18.095619788662866 70-71 54.02586484980596 16.071201650032744 12.274236485143513 17.628697015017774 72-73 54.63011234783516 15.135025936449184 11.982248520710058 18.252613195005605 74-75 55.96035229372147 14.10250144182364 12.202226762201738 17.734919502253156 76 58.15900944933203 11.947865754317368 12.183121537960249 17.710003258390355 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.5 6 0.5 7 0.0 8 0.0 9 0.5 10 0.5 11 1.0 12 2.0 13 5.5 14 9.0 15 10.0 16 14.0 17 17.0 18 21.5 19 38.5 20 56.5 21 62.0 22 88.5 23 137.0 24 172.0 25 185.0 26 257.5 27 360.0 28 464.5 29 539.0 30 652.5 31 769.0 32 952.5 33 1133.0 34 1216.5 35 1393.5 36 1534.5 37 1582.0 38 1812.5 39 2053.0 40 2240.5 41 2484.5 42 2551.0 43 2552.0 44 2604.5 45 2738.0 46 2820.0 47 2872.0 48 3261.5 49 3580.0 50 3561.0 51 2960.0 52 2240.5 53 2044.0 54 1966.0 55 1925.0 56 1998.5 57 2104.5 58 2096.0 59 2204.5 60 2457.5 61 2730.0 62 2858.0 63 2883.0 64 3095.5 65 3297.0 66 3383.0 67 3455.0 68 3340.0 69 3184.5 70 3012.5 71 2881.0 72 2858.5 73 2783.5 74 2669.5 75 2608.0 76 2424.0 77 2112.0 78 1802.5 79 1621.0 80 1528.0 81 1258.0 82 943.5 83 806.0 84 732.5 85 559.0 86 452.0 87 445.0 88 420.0 89 419.0 90 474.0 91 531.0 92 557.0 93 610.5 94 798.0 95 1083.0 96 1234.0 97 1634.0 98 3024.0 99 24564.0 100 45114.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 6.516695774574459E-4 5 0.0 6 0.0 7 0.0026066783098297837 8 6.516695774574459E-4 9 0.0 10-11 3.2583478872872296E-4 12-13 0.0 14-15 0.0 16-17 0.0 18-19 3.2583478872872296E-4 20-21 0.0 22-23 6.516695774574459E-4 24-25 3.2583478872872296E-4 26-27 0.0 28-29 0.0 30-31 3.2583478872872296E-4 32-33 3.2583478872872296E-4 34-35 3.2583478872872296E-4 36-37 6.516695774574459E-4 38-39 3.2583478872872296E-4 40-41 3.2583478872872296E-4 42-43 0.0 44-45 3.2583478872872296E-4 46-47 6.516695774574459E-4 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0013033391549148918 64-65 6.516695774574459E-4 66-67 3.2583478872872296E-4 68-69 3.2583478872872296E-4 70-71 3.2583478872872296E-4 72-73 0.0 74-75 3.2583478872872296E-4 76 0.0013033391549148918 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 153452.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.96254203268774 #Duplication Level Percentage of deduplicated Percentage of total 1 80.75571645327015 39.540051612230535 2 9.294061277184763 9.10121731877069 3 5.058961322437246 7.430988191747256 4 2.5660819336119465 5.025675781351823 5 1.1526073415497644 2.821729270390741 6 0.5150797242260495 1.5131767588561895 7 0.23025527723800143 0.789171858300967 8 0.11978598237815104 0.4692020957693611 9 0.07187158942689062 0.31671141464431873 >10 0.2089599914818857 1.9986705940619867 >50 0.011978598237815104 0.41967520788259527 >100 0.01064764287805787 0.9051690430883925 >500 0.0013309553597572338 0.35515991971430805 >1k 0.0013309553597572338 1.3672027735057217 >5k 0.0 0.0 >10k+ 0.0013309553597572338 27.946198159685114 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 42884 27.946198159685114 No Hit CTTATACACATCTGACGCTGCCGACGAGGGCCGGTGTGTAGATCTCGGTG 2098 1.3672027735057217 No Hit CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 545 0.35515991971430805 No Hit GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 285 0.18572582957537212 No Hit GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 234 0.15249068112504235 No Hit TCTTATACACATCTGACGCTGCCGACGAGGGCCGGTGTGTAGATCTCGGT 197 0.12837890675911687 No Hit GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 165 0.10752548028047859 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 6.51669577457446E-4 0.0 3 0.0 0.0 0.0 6.51669577457446E-4 0.0 4 0.0 0.0 0.0 0.001955008732372338 0.0 5 0.0 0.0 0.0 0.005213356619659568 0.0 6 0.0 0.0 0.0 0.0065166957745744596 0.0 7 0.0 0.0 0.0 0.0065166957745744596 0.0 8 0.0 0.0 0.0 0.007820034929489352 0.0 9 0.0 0.0 0.0 0.008471704506946797 0.0 10 0.0 0.0 0.0 0.008471704506946797 0.0 11 0.0 0.0 0.0 0.010426713239319136 0.0 12 0.0 0.0 0.0 0.012381721971691474 0.0 13 0.0 0.0 0.0 0.012381721971691474 0.0 14 0.0 0.0 0.0 0.014336730704063811 0.0 15 0.0 0.0 0.0 0.014336730704063811 0.0 16 0.0 0.0 0.0 0.014336730704063811 0.0 17 0.0 0.0 0.0 0.014336730704063811 0.0 18 0.0 0.0 0.0 0.01759507859135104 0.0 19 0.0 0.0 0.0 0.023460104788468054 0.0 20 0.0 0.0 0.0 0.028021791830670176 0.0 21 0.0 0.0 0.0 0.03714516591507442 0.0 22 0.0 0.0 0.0 0.05017855746422334 0.0 23 0.0 0.0 0.0 0.052133566196595676 0.0 24 0.0 0.0 0.0 0.06581862732320204 0.0 25 0.0 0.0 0.0 0.06972864478794671 0.0 26 0.0 0.0 0.0 0.07689701013997863 0.0 27 0.0 0.0 0.0 0.10296379323827647 0.0 28 0.0 0.0 0.0 0.15379402027995726 0.0 29 0.0 0.0 0.0 0.2052759168990955 0.0 30 0.0 0.0 0.0 0.2782629095743294 0.0 31 0.0 0.0 0.0 0.3362615019680421 0.0 32 0.0 0.0 0.0 0.42749524281208456 0.0 33 0.0 0.0 0.0 0.5298073664729036 0.0 34 0.0 0.0 0.0 0.7370382921043714 0.0 35 0.0 0.0 0.0 0.9801110444959987 0.0 36 0.0 0.0 0.0 1.3085525115345515 0.0 37 0.0 0.0 0.0 1.8142481036415297 0.0 38 0.0 0.0 0.0 2.238484998566327 0.0 39 0.0 0.0 0.0 2.6412167974350287 0.0 40 0.0 0.0 0.0 3.1351823371477727 0.0 41 0.0 0.0 0.0 3.58744102390324 0.0 42 0.0 0.0 0.0 4.07749654615124 0.0 43 0.0 0.0 0.0 4.597528868962281 0.0 44 0.0 0.0 0.0 5.082371034590621 0.0 45 0.0 0.0 0.0 5.559393165289472 0.0 46 0.0 0.0 0.0 6.072257122748481 0.0 47 0.0 0.0 0.0 6.527774157391236 0.0 48 0.0 0.0 0.0 6.91225920809113 0.0 49 0.0 0.0 0.0 7.3091259807627145 0.0 50 0.0 0.0 0.0 7.670802596251597 0.0 51 0.0 0.0 0.0 8.015535802726586 0.0 52 0.0 0.0 0.0 8.44042436722884 0.0 53 0.0 0.0 0.0 8.792977608633318 0.0 54 0.0 0.0 0.0 9.14487918046034 0.0 55 0.0 0.0 0.0 9.569767744962594 0.0 56 0.0 0.0 0.0 9.936006047493679 0.0 57 0.0 0.0 0.0 10.289210958475614 0.0 58 0.0 0.0 0.0 10.605270703542477 0.0 59 0.0 0.0 0.0 10.882881943539347 0.0 60 0.0 0.0 0.0 11.178739931705028 0.0 61 0.0 0.0 0.0 11.496754685504262 0.0 62 0.0 0.0 6.51669577457446E-4 11.78544430831791 0.0 63 0.0 0.0 6.51669577457446E-4 12.115840784088835 0.0 64 0.0 0.0 6.51669577457446E-4 12.479472408310091 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCAAGA 15 0.002218639 70.02053 8 AGAACCG 15 0.0022215096 69.99772 12 TTGTGCG 25 2.3848751E-4 55.998177 2 GTTAGTG 20 0.0069325697 52.515404 7 CCAGTTG 20 0.0069325697 52.515404 9 AACGTGA 20 0.006941514 52.49829 46 CTGACAT 20 0.006941514 52.49829 43 GTAGATC 355 0.0 49.29417 38 GTATCAT 350 0.0 48.998405 57 TCTCGGT 365 0.0 48.902515 43 GCCGTAT 340 0.0 48.380775 54 AGATCTC 370 0.0 48.241673 40 CGCCGTA 350 0.0 47.99844 53 ATCATTA 350 0.0 47.99844 59 TAGATCT 365 0.0 47.94364 39 TGGTCGC 365 0.0 47.94364 49 CCGTATC 345 0.0 47.679607 55 CATTAAA 360 0.0 47.637333 61 TATCATT 355 0.0 47.322403 58 CGTATCA 355 0.0 47.322403 56 >>END_MODULE