FastQCFastQC Report
Wed 25 May 2016
SRR1781284_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781284_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences153452
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT66974.3642311602325154No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT66044.303625889528973No Hit
CAAATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT41032.673800276307901No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG36512.379245627297135No Hit
CATATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT27081.7647212157547636No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG21531.4030446002658812No Hit
TCATATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG18371.1971170137893283No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC12840.8367437374553607No Hit
CACTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC4640.30237468394025496No Hit
CTAATACCCATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT3970.25871282225060604No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC3810.24828610901128692No Hit
CAAATACCCATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT3770.24567943070145712No Hit
CAAAAACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT3500.2280843521101061No Hit
CTAAAACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT3220.20983760394129763No Hit
TCATTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC3170.20657925605401037No Hit
CTAATACACATCTCCGCGCCCACGAGACTTGCTAAGATCTCGTATGCCGT2770.18051247295571254No Hit
CTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTC2310.15053567239267002No Hit
CTTATACACATCTCCGCGCCCACGAGACTTGCTAAGATCTCGTATGCCGT2310.15053567239267002No Hit
CTTATACCCATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT2270.14792899408284024No Hit
AAAATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT2210.14401897661809557No Hit
CAAATACACATCTCCGCGCCCACGAGACTTGCTAAGATCTCGTATGCCGT2140.13945728957589346No Hit
CATATACCCATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT1740.1133905064775956No Hit
ATAATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT1640.10687381070302114No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAA252.8108407E-670.07699648
CGAATTC150.002211553770.07699648
TATGCAT252.82176E-670.031353
TCAGCTA207.8892765E-570.031353
GAATAGC207.914804E-569.9856626
TCACGTT150.002223027669.9856623
CAAATAC8850.064.054671
AGGGGGG22000.063.6233370
CTAATAC11200.062.1747671
AAATACA9400.062.1681142
ATCGTAT1250.061.58738739
AATACAC20550.061.3013153
AATGACG401.0524673E-861.29737543
CGAATGA401.0524673E-861.29737541
AATACCC1950.061.013143
AAATACC1100.060.442162
TAATACA11350.060.428152
CCGTCTA1050.060.06599847
AAGACGT352.9430885E-760.04640644
AGCTAGA352.9564035E-760.00726355
Produced by FastQC (version 0.11.5)