FastQCFastQC Report
Wed 25 May 2016
SRR1781283_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781283_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21102
Sequences flagged as poor quality0
Sequence length76
%GC68

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG390418.500616055350203No Hit
CTTATACACATCTGACGCTGCCGACGAGGGCCGGTGTGTAGATCTCGGTG8293.9285375793763624No Hit
TCTTATACACATCTGACGCTGCCGACGAGGGCCGGTGTGTAGATCTCGGT930.4407165197611601No Hit
ATACACATCTGACGCTGCCGACGAGGGCCGGTGTGTAGATCTCGGTGGTC530.2511610273907687No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG480.2274665908444697No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGGCCGGTGTGTAGATCTCGG480.2274665908444697No Hit
TCTTTATACACATCTGACGCTGCCGACGAGGGCCGGTGTGTAGATCTCGG410.1942943796796512No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG260.12321107004075443No Hit
CTATACACATCTGACGCTGCCGACGAGGGCCGGTGTGTAGATCTCGGTGG220.1042555208037153No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACTAC150.002194676570.048
TAGATCT1850.043.51351539
GTGTAGA1950.043.07692336
TGTAGAT1950.043.07692337
GTAGATC1900.042.36842338
AAAAAGG756.8394E-1042.070
AGATCTC1850.041.6216240
GTGTGTA1850.041.6216234
GTATCAT1950.041.2820557
CCGTATC1950.041.2820555
CGTATCA1950.041.2820556
ATCATTA1950.041.2820559
GCCGTAT1950.041.2820554
TCATTAA1950.041.2820560
CGCCGTA1950.041.2820553
TGTGTAG1950.041.2820535
CATTAAA2050.040.9756161
GGTGTGT1800.040.83333233
TCGGTGG1900.040.52631845
TCTCGGT1900.040.52631843