FastQCFastQC Report
Wed 25 May 2016
SRR1781283_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781283_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21102
Sequences flagged as poor quality0
Sequence length76
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17518.297791678513885No Hit
CAAATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT1070.5070609420907971No Hit
AAAATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT420.199033266988911No Hit
TCATTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC420.199033266988911No Hit
CTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTC400.18955549237039143No Hit
TCATATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG360.17059994313335228No Hit
CAATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTC250.11847218273149465No Hit
CTAATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT230.10899440811297507No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAGCAT207.6743876E-570.1377753
CGTCTAC150.002177556370.1377748
ACTCGTA207.765531E-569.97156538
TCGAATG150.002198215869.97156540
ACGTATG150.002198215869.97156540
GGATCAC150.002198215869.97156535
ATCACGT150.002198215869.97156537
GGGAAGT150.002198215869.9715654
AGATCTC150.002198215869.97156535
AAAATAC800.061.225121
AAATACA1800.058.3096352
ACCGAGC900.058.30963513
TAGTATG308.119243E-658.30963540
CGGAACT308.119243E-658.30963534
AGCATGA308.119243E-658.30963555
CGAGACG308.119243E-658.30963523
AAAAACC308.119243E-658.3096352
ACATCAG252.3147398E-456.11021850
ACTCCGA509.094947E-1055.97725711
AACTCGT252.3421645E-455.97725737