FastQCFastQC Report
Wed 25 May 2016
SRR1781282_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781282_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences188013
Sequences flagged as poor quality0
Sequence length76
%GC74

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5644430.021328312403927No Hit
CTTATACACATCTGACGCTGCCGACGAGGGCCGGTGTGTAGATCTCGGTG34901.8562546206911223No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6400.3404019934791743No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4410.23455824863174357No Hit
TCTTATACACATCTGACGCTGCCGACGAGGGCCGGTGTGTAGATCTCGGT3430.18243419338024497No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2950.15690404386930693No Hit
GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2450.13031013812874642No Hit
CTCTTATACACATCTGACGCTGCCGACGAGGGCCGGTGTGTAGATCTCGG1950.10371623238818592No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCTAA150.002222002770.000011
GGTTAAG150.002222002770.000011
AAGCTAC150.002222002770.0000164
CGACAGT252.385878E-456.00000458
TCGATGA200.006943040552.517
TATTGTG200.006943040552.546
TCTACGA200.006943040552.52
GTTAAGA200.006943040552.52
TAATTCT200.006943040552.541
GTCTTGA200.006943040552.516
GACTGTG200.006943040552.57
TGTCGGT200.006943040552.543
CTGGCAA200.006943040552.556
CGTATCA5400.051.85185256
GCCGTAT5300.051.5094354
CCGTATC5350.051.02803455
ATCATTA5400.050.55555359
TATCATT5500.050.27272858
TATAAGA352.0807554E-550.02
ATCTCGG5550.049.8198242
Produced by FastQC (version 0.11.5)