FastQCFastQC Report
Wed 25 May 2016
SRR1781282_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781282_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences188013
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT92594.924659465036992No Hit
CTAATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT86274.588512496476308No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG59663.1731848329636785No Hit
CAAATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT51692.7492779754591434No Hit
CATATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT34731.8472126927393318No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG25331.3472472648167946No Hit
TCATATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG23551.2525729603803992No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC19531.038757958226293No Hit
CACTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC6790.36114523995681147No Hit
CTAATACCCATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT5950.31646747831266986No Hit
CAAATACCCATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT4910.26115215437230405No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC4060.2159425146133512No Hit
CAAAAACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT3870.20583683043193823No Hit
TCATTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC3510.18668921829873467No Hit
CTTATACCCATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT3440.1829660714950562No Hit
CTAAAACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT3380.17977480280618893No Hit
CTAATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGACGT3190.16966911862477593No Hit
CTAATACACATCTCCGCGCCCACGAGACAGTAGATTATCTCGTATGCCGT3010.16009531255817416No Hit
AAAATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT2750.1462664815730827No Hit
CAAATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGACGT2590.13775643173610336No Hit
CTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTC2450.13031013812874642Illumina PCR Primer Index 10 (95% over 21bp)
CATATACCCATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT2400.12765074755469036No Hit
CTTATACACATCTCCGCGCCCACGAGACAGTAGATTATCTCGTATGCCGT2290.12180008829176706No Hit
CAAATACACATCTCCGCGCCCACGAGACAGTAGATTATCTCGTATGCCGT2130.11329003845478772No Hit
ATAATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT2090.11116252599554287No Hit
CATAAACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT1930.1026524761585635No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACTAA150.002216680470.0423348
AATCCGT207.887745E-570.0423244
ATCCGTC207.887745E-570.0423245
TAATCCG252.8216E-670.0423243
GTTAGCC150.00221902270.0236842
ACTAATG207.898154E-570.0236850
ATGGTTC150.002221365770.005067
ATCGTAA207.908573E-570.0050539
TCGTAAT207.908573E-570.0050540
AAACTCG150.00222371169.98643536
ATAGGTC150.00222371169.98643569
GTTTAGA150.00222371169.98643526
AGCGGTA150.00222371169.98643512
GCTAATC150.00222371169.98643533
CGTTTAT150.00222371169.98643533
AGGGGGG23950.065.6031670
AATACCC2400.062.712863
CTAATAC15200.062.4058231
AATACAC28350.061.3624573
TCTTTAT800.061.254421
Produced by FastQC (version 0.11.5)