FastQCFastQC Report
Wed 25 May 2016
SRR1781281_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781281_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences219153
Sequences flagged as poor quality0
Sequence length76
%GC69

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4615721.061541480153135No Hit
CTTATACACATCTGACGCTGCCGACGAGGGCCGGTGTGTAGATCTCGGTG22601.0312430128722856No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6520.29750904619147356No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3280.14966712753190695No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2580.11772597226595118No Hit
TCTTATACACATCTGACGCTGCCGACGAGGGCCGGTGTGTAGATCTCGGT2380.10859992790424955No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCCCG150.002222495970.054
CCAATCT150.002222495970.09
TTGGGCG150.002222495970.02
TATGGAC200.006944569352.53
ACTTAAC200.006944569352.537
CGTATCA3400.049.41176656
GCCGTAT3400.049.41176654
CCGTATC3450.048.69565255
GTAGATC3600.047.63888538
TAGATCT3550.047.32394439
ATCATTA3500.047.059
TGTCATT305.868936E-446.66666424
CAATTAC305.868936E-446.66666468
GTATCAT3700.046.35135357
TATCATT3600.045.69444358
TCTCGGT3700.045.40540743
TCATTAA3550.045.35211660
AGATCTC3600.044.7222240
CATTAAA3400.044.26470661
TGTAGAT4150.043.0120537
Produced by FastQC (version 0.11.5)