FastQCFastQC Report
Wed 25 May 2016
SRR1781281_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781281_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences219153
Sequences flagged as poor quality0
Sequence length76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT87263.9816931550104266No Hit
CTAATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT68573.128864309409408No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG40351.8411794499733063No Hit
CAAATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT39241.7905299037658622No Hit
CATATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT30761.4035856228297126No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG22771.039000150579732No Hit
TCATATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG18050.8236255036435732No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC17900.816780970372297No Hit
CACTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC5770.26328637983509234No Hit
CTAATACCCATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT3800.1733948428723312No Hit
CAAATACCCATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT3550.16198728742020416No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC3530.161074682984034No Hit
TCATTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC3110.14190998982446051No Hit
CAAAAACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT3000.13689066542552464No Hit
CTTATACCCATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT2900.1323276432446738No Hit
CTAAAACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT2670.12183269222871693No Hit
CTAATACACATCTCCGCGCCCACGAGACGTGATAGCATCTCGTATGCCGT2330.10631841681382415No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAA150.002214142870.0664746
CCTTAAT150.002224191669.98653467
ATCGTAA150.002224191669.98653439
AGGGGGG21450.062.31901670
AATACAC23500.061.498813
AAATACA11500.060.8578572
CAAATAC10750.060.5464941
CTAATAC12500.059.9084741
TAATACA12700.058.68952
AATACCC2050.058.0376173
GTAAGCC2050.056.39496242
CCGTAAT252.3755833E-456.05317347
AACCGAG252.389024E-455.98922712
CAAGTAT252.389024E-455.98922739
GCATATC1000.055.98922735
TAAGCCG1950.055.6938643
TATCGTA1200.055.40600638
ATCGTAT1150.054.77206839
GCATGAA4600.054.01134556
CGTAAGC2300.053.27493341
Produced by FastQC (version 0.11.5)