FastQCFastQC Report
Wed 25 May 2016
SRR1781280_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781280_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences210871
Sequences flagged as poor quality0
Sequence length76
%GC69

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5042323.911775445651607No Hit
CTTATACACATCTGACGCTGCCGACGAGGGCCGGTGTGTAGATCTCGGTG28741.362918561585045No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5510.2612971911737508No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3820.1811534065850686No Hit
TCTTATACACATCTGACGCTGCCGACGAGGGCCGGTGTGTAGATCTCGGT3250.15412266267054267No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2790.13230837810794277No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTGC252.386547E-455.9999961
CCGTATC4600.053.2608755
GATACGG200.006944205652.541
TTACTCG200.006944205652.519
CTAAACG200.006944205652.544
CGATACG200.006944205652.540
CCATTCA200.006944205652.539
GCCGTAT4750.052.31579254
CGTATCA4650.051.93548256
GTATCAT4900.050.71428357
ATCATTA4900.050.71428359
TATCATT5000.050.39999858
TAGATCT5000.050.39999839
TCTCGGT5100.050.09803843
CGCCGTA5050.049.9009953
TGGTCGC5200.049.8076949
TCATTAA5000.049.69999760
GTAGATC5100.049.41176238
TGTAGAT5200.047.7884637
ATCTCGG5250.047.33333242
Produced by FastQC (version 0.11.5)