FastQCFastQC Report
Wed 25 May 2016
SRR1781280_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781280_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences210871
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT75453.5780168918438284No Hit
CAAATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT60582.872846432178915No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT50062.3739632287038046No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG48392.294767891270018No Hit
CATATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT28001.327826016853906No Hit
TCATATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG26411.252424468039702No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG22471.0655803785252596No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC10980.5206974880377102No Hit
CAAATACCCATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT6780.32152358550962434No Hit
CTAATACCCATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT6040.2864310407784854No Hit
CACTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC5570.26414253263843773No Hit
CAAAAACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT4200.19917390252808587No Hit
TCATTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC3810.18067918300762076No Hit
AAAATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT3660.17356582934590342No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC3500.16597825210673825No Hit
CTAAAACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT2940.13942173176966013No Hit
CAAATACACATCTCCGCGCCCACGAGACTACTTGAAATCTCGTATGCCGT2550.12092701224919501No Hit
CTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTC2370.11239098785513418TruSeq Adapter, Index 12 (95% over 21bp)
CTAATACACATCTCCGCGCCCACGAGACTACTTGAAATCTCGTATGCCGT2320.11001986996789506No Hit
CTTATACCCATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT2200.10432918703852118No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACCGA150.002224888769.9800811
AATACCC2650.066.018943
CAAATAC13300.063.9291731
AAATACA13300.063.1399232
AAATACC2000.062.9820752
AGGGGGG21050.060.83778870
AAAATAC1900.060.7721751
AATACAC26600.060.7721753
CTAATAC13350.058.709881
CGTCTTA1200.058.39982648
CCGTAAT308.300209E-658.39982647
TATGCAT308.335337E-658.3582553
AAATCGT308.358826E-658.33056337
TAATACC900.058.316732
AACTCGT1900.057.1025537
TAATACA13700.056.954592
TCTTTAT800.056.8588141
CCGACTA252.3731434E-456.06383547
ACAACTC3750.055.0509958
AACTCCG3650.054.6419810
Produced by FastQC (version 0.11.5)