FastQCFastQC Report
Wed 25 May 2016
SRR1781279_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781279_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences207977
Sequences flagged as poor quality0
Sequence length76
%GC71

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5112224.580602662794444No Hit
CTTATACACATCTGACGCTGCCGACGAGGGCCGGTGTGTAGATCTCGGTG24331.169840895868293No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5850.2812811031989114No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4810.23127557374132715No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2820.13559191641383422No Hit
TCTTATACACATCTGACGCTGCCGACGAGGGCCGGTGTGTAGATCTCGGT2230.10722339489462777No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTGCG407.454473E-752.52
CATTGAT200.00694407252.542
GATTTGC200.00694407252.564
CCATGTA200.00694407252.55
GTAATGG200.00694407252.562
TGCGTGA200.00694407252.541
GCCGTAT4800.047.39583254
CGTATCA4900.047.14285756
TCATTAA5000.046.89999860
TCTCGGT4950.046.66666443
GTATCAT5100.046.66666457
TATCATT5000.046.258
CCGTATC4850.046.18556655
ATCTCGG5200.045.09615342
ATCATTA5200.045.09615359
CATTAAA5250.044.66666861
AGATCTC5350.044.4859840
TGGTCGC5350.044.4859849
TAGATCT5400.044.07407439
CGCGTGT404.581771E-543.757