FastQCFastQC Report
Wed 25 May 2016
SRR1781279_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781279_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences207977
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT88794.269222077441256TruSeq Adapter, Index 5 (95% over 23bp)
CTAATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT70253.3777773503800903TruSeq Adapter, Index 5 (95% over 23bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG42932.0641705573212423No Hit
CAAATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT39621.9050183433745076TruSeq Adapter, Index 5 (95% over 23bp)
CATATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT29411.4140986743726471TruSeq Adapter, Index 5 (95% over 23bp)
TCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG23861.147242243132654TruSeq Adapter, Index 5 (95% over 22bp)
TCATATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG18210.875577587906355TruSeq Adapter, Index 5 (95% over 22bp)
CTCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC15500.7452747178774576RNA PCR Primer, Index 5 (95% over 21bp)
CTAATACACATCTCCGCGCCCACGAGACACAAAGTGATCTCGTATGCCGT4790.23031392894406594TruSeq Adapter, Index 5 (95% over 23bp)
CACTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC4770.22935228414680467TruSeq Adapter, Index 5 (95% over 21bp)
CTTATACACATCTCCGCGCCCACGAGACACAAAGTGATCTCGTATGCCGT4070.19569471624266144TruSeq Adapter, Index 5 (95% over 23bp)
CTAATACCCATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT3900.18752073546594095TruSeq Adapter, Index 5 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC3330.16011385874399572RNA PCR Primer, Index 5 (95% over 21bp)
CAAAAACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT3120.15001658837275275RNA PCR Primer, Index 5 (95% over 23bp)
CTAAAACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT3030.1456891867850772No Hit
CAAATACACATCTCCGCGCCCACGAGACACAAAGTGATCTCGTATGCCGT2970.14280425239329347TruSeq Adapter, Index 5 (95% over 23bp)
CAAATACCCATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT2940.14136178519740164TruSeq Adapter, Index 5 (95% over 23bp)
CTTATACCCATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT2680.1288604028330056TruSeq Adapter, Index 5 (95% over 23bp)
TCATTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC2660.12789875803574435No Hit
CTAATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGACGT2200.10578092769873591No Hit
AAAATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT2110.10145352611106036No Hit
CAAATACACATCTCCGAGCCCACGAGACACAAAGTGATCACGTATGCCGT2090.10049188131379912No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACTT150.00221773570.03657553
ATACGAG150.00221773570.03657554
GACGAAT150.00221773570.03657546
AATACGA150.00221773570.03657553
ACGTAAG550.070.00288440
TATCGAA150.002224092669.98604638
CTAATAC14000.065.50271
AATACCC2300.065.437483
TAATACA14000.064.252652
AATACAC24400.063.5477033
CAAATAC9950.062.263871
CCGTAAT900.062.2547347
AGGGGGG23700.062.16061470
AAATACA10800.059.9561772
CGTCTAA308.330231E-658.3638148
ACGTCTA308.330231E-658.3638147
CGACTAA308.330231E-658.3638148
CAAGTAT308.354033E-658.33573539
CACGTAA601.8189894E-1258.33573539
ATCGAAT308.354033E-658.33573539
Produced by FastQC (version 0.11.5)