FastQCFastQC Report
Wed 25 May 2016
SRR1781278_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781278_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences315880
Sequences flagged as poor quality0
Sequence length76
%GC72

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9772130.93611498037229No Hit
CTTATACACATCTGACGCTGCCGACGATAAACTGGGTGTAGATCTCGGTG28340.8971761428390528No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11040.3494998100544511No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7340.23236672153982524No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4840.15322274281372672No Hit
GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3880.12283145498290489No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCGA150.002223406770.025
CCCTACG200.006947393552.546
GACGTAT200.006947393552.554
CGATTGT200.006947393552.56
TGATTCG200.006947393552.532
ACTACTT200.006947393552.521
TATGTTC200.006947393552.57
GCCGTAT5100.050.78431354
CCGTATC5150.049.6116555
CGTATCA5250.049.33333256
TAGATCT5250.049.33333239
TCTCGGT5650.048.9380543
TATCATT5400.047.96296358
TGTAGAT5850.047.86324737
GTAGATC5500.047.72727238
ATCATTA5450.047.52293859
TGGTCGC6000.047.24999649
GTATCAT5550.046.66666857
AGCCGTT305.872894E-446.66666428
CGCCGTA5750.046.26086853
Produced by FastQC (version 0.11.5)