FastQCFastQC Report
Wed 25 May 2016
SRR1781278_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781278_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences315880
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT266338.431366341648728No Hit
CATATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT201186.3688742560466No Hit
CAAATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT73302.320501456249209No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG65622.077371153602634No Hit
CTAATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT54601.7285044953779916No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG48441.5334937317968849No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC36971.170381157401545No Hit
CACTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC23340.7388881853868557No Hit
TCATATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG20070.635367861213119No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC9200.29124984171204255No Hit
ATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTCTT5820.18424718247435737RNA PCR Primer, Index 48 (95% over 23bp)
CATATAAACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT5660.17918196783588705No Hit
CATATACACATCTCCGAGCCCACGAGACTTTCGTCAATATCGTATGCCGT5220.1652526275800937No Hit
CATATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGACGT4500.14245916170697734No Hit
CTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTC4230.1339116120045587No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATATCGTATGCCGT3720.1177662403444346No Hit
CAAATAAACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT3600.11396732936558188No Hit
CATATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCAGT3500.11080157021653791No Hit
CAAATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGACGT3440.10890211472711156No Hit
CAAATACACATCTCCGAGCCCACGAGACTTTCGTCAATATCGTATGCCGT3260.10320374825883247No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACTTA301.0163967E-770.0543548
TCCATAG150.002224942769.98781631
ACTAACG207.926564E-569.98781627
CAAATAC15100.066.51161
AGGGGGG54100.064.8131170
AAATACA15900.064.705712
ACGTAAG600.064.15549540
CCGTAAT550.063.68577247
GCCGTAA453.8198777E-1062.2705346
CGTAAGA401.0613803E-861.26843341
AATACAC26500.060.876193
AAACTCG2250.060.65610536
ACTCGTA2050.059.7456938
AAACATC6600.059.3835956
AATCGTA601.8189894E-1258.32317738
ATATACA34150.057.896212
TAAACAT6800.057.6370245
CATATAC34150.057.58881
AACTCGT2200.057.26275637
ATTCGTC1600.056.86509729
Produced by FastQC (version 0.11.5)