FastQCFastQC Report
Wed 25 May 2016
SRR1781277_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781277_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences198895
Sequences flagged as poor quality0
Sequence length76
%GC79

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7778239.107066542648134No Hit
CTTATACACATCTGACGCTGCCGACGATAAACTGGGTGTAGATCTCGGTG23261.169461273536288No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8540.4293722818572614No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5890.29613615224113227No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4100.2061389175192941No Hit
GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3420.17195002388194774No Hit
GGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2510.12619723974961664No Hit
GGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2400.12066668342592825No Hit
TCTTATACACATCTGACGCTGCCGACGATAAACTGGGTGTAGATCTCGGT2370.11915834988310416No Hit
GGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2120.10658890369290329No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTAT150.00222219270.0000154
TCTGTTA150.00222219270.0000125
TAGACGC308.354738E-658.3333363
AAACTTC200.006943627352.539
TCGTTTG200.006943627352.521
GCAGTAT200.006943627352.554
GTGTGTA200.006943627352.534
GCCTAGC200.006943627352.540
CGTGTGC200.006943627352.51
TGTCCAT200.006943627352.535
TCATTAA4100.050.36585260
CTTGTGC707.2759576E-1250.01
TATCATT4100.048.6585458
ATCATTA4250.048.58823459
GCCGTAT4050.048.3950654
GTATCAT4400.047.72727257
CCGTATC4150.047.22891255
CGTATCA4300.047.209356
TTGTGCG751.4551915E-1146.6666682
AGTATCA305.8676157E-446.66666856
Produced by FastQC (version 0.11.5)