FastQCFastQC Report
Wed 25 May 2016
SRR1781277_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781277_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences198895
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT1996110.035948616103974No Hit
CATATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT161598.124387239498228No Hit
CAAATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT60113.022197641971895No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG47912.4088086678900926No Hit
CTAATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT42432.13328640740089No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG40242.0231780587747306No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC30051.5108474320621434No Hit
CACTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC19210.9658362452550341No Hit
TCATATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG16950.8522084516956183No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC7140.3589833831921366No Hit
CATATAAACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT4630.2327861434425199No Hit
ATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCTT4440.22323336433796728TruSeq Adapter, Index 2 (95% over 21bp)
CATATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGACGT4140.20815002890972623No Hit
CTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTC3520.1769778023580281No Hit
CAAATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGACGT3380.16993891249151563No Hit
CATATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCAGT3240.16290002262500314No Hit
CAAATAAACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT3200.160888911234571No Hit
CATATACACATCTCCGAGCCCACGAGACGCGCACGTATCTCGTATGCCGT2790.1402750194826416No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGACGT2660.13373890746373715No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGCACGTATCTCGTATGCCGT2620.131727796073305No Hit
CAATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTC2390.12016390557832023No Hit
TCATTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC2320.11664446064506397No Hit
CAAATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCAGT2290.11513612710223987No Hit
CTAATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGACGT2260.11362779355941577No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCAGT2180.1096055707785515No Hit
CTTATAAACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT2180.1096055707785515No Hit
AAACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCTT2120.10658890369290329RNA PCR Primer, Index 2 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGAGCAAGTATCTCGTATGCCGT1990.10005279167399883No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACGTA150.00221330470.0707445
ATACCGT252.8154027E-670.0707444
GTTTAAT150.002215515470.0531141
GTATACC252.8196173E-670.0531142
ATTCAGA150.002217728670.0354851
CTCGAAA455.456968E-1270.00024439
AAACTCG401.3460522E-1069.9826536
AATTTGC150.00222437869.9826564
AATCACG150.00222437869.9826536
GTTTCGT150.00222437869.982656
TAACGAA150.00222437869.9826538
TAGCACG207.922248E-569.9826529
ATATCAG207.922248E-569.9826510
CGAATCA207.922248E-569.9826534
GTATAAC353.6998244E-969.9826535
TCTTTAT1100.069.982651
ATAACGA150.00222437869.9826537
TAAATCG207.922248E-569.9826536
CGTTTCG150.00222437869.982655
TATAACG301.02169E-769.9826536
Produced by FastQC (version 0.11.5)