FastQCFastQC Report
Wed 25 May 2016
SRR1781276_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781276_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences318644
Sequences flagged as poor quality0
Sequence length76
%GC67

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7489923.505542235221753No Hit
CTTATACACATCTGACGCTGCCGACGATAAACTGGGTGTAGATCTCGGTG20280.6364469439248818No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9370.29405857320395173No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7440.23348941138072585No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3690.11580321612834386No Hit
GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3310.10387768167610248No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAT4250.052.70588354
ATTACGC200.006947450352.49999612
TTATCGG200.006947450352.49999625
GTGCGAA200.006947450352.4999969
CGTATCA4300.052.09302556
GTATCAT4200.050.83333257
TAGATCT4500.050.55555739
CCGTATC4400.050.11363655
GTAGATC4550.050.038
TCTCGGT4600.049.4565243
CGCCGTA4550.049.2307753
TATCATT4200.049.16666458
TGGTCGC4850.049.07216649
CGGTGGT4950.048.78787646
ATCATTA4500.046.66666859
AGATCTC4800.046.66666440
TCGGTGG5150.046.2135945
TCATTAA4650.045.1612960
GATCTCG5000.044.841
TGTAGAT5100.044.60784537
Produced by FastQC (version 0.11.5)