FastQCFastQC Report
Wed 25 May 2016
SRR1781276_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781276_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences318644
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT204946.431629028006176No Hit
CATATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT148584.6628839708263765No Hit
CAAATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT57241.7963620843323584No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG49081.5402769234631752No Hit
CTAATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT44301.3902662532481391No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG36401.142340668583121No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC26510.8319629429708388No Hit
TCATATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG16380.5140533008624044No Hit
CACTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC15360.48204265575375654No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC7370.23129260240268137No Hit
ATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCTT4760.14938301050702352RNA PCR Primer, Index 47 (95% over 21bp)
CATATAAACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT4030.12647343116455984No Hit
CATATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGACGT3710.11643087583635657No Hit
CTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTC3430.1076436399241787No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTAT207.889618E-570.0540446
ATCGTAA252.8365048E-669.99905439
TTGACGG150.002224927569.98807519
CAAATAC12250.064.274761
AGGGGGG39250.064.1928870
AAATACA13500.060.1378972
AATACAC22750.058.759223
CGTATGA3800.058.0346441
TAAGACG509.731593E-1056.03442443
CATATAC26200.055.4294851
CTAATAC9650.055.120141
ATATACA27750.054.2249952
ATATCGT3450.053.7673937
TATGACG4200.053.3661243
TATCGTA3750.053.19928438
ATCGTAT3800.052.49929439
GTCGTGT200.006952132652.49105512
CTACCCG200.006952132652.49105519
ATTGACG200.006952132652.49105518
GACGTCT3700.052.0671946
Produced by FastQC (version 0.11.5)