FastQCFastQC Report
Wed 25 May 2016
SRR1781275_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781275_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences193473
Sequences flagged as poor quality0
Sequence length76
%GC86

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9661149.93513306766319No Hit
CTTATACACATCTGACGCTGCCGACGATAAACTGGGTGTAGATCTCGGTG31461.6260666863076503No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11240.5809596171041954No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8390.4336522408811565No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5400.2791087128436526No Hit
GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4650.24034361383758973No Hit
TCTTATACACATCTGACGCTGCCGACGATAAACTGGGTGTAGATCTCGGT3220.16643149173269656No Hit
GGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3120.1612628118652215No Hit
GGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3090.15971220790497898No Hit
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3020.15609413199774647No Hit
GGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2420.12508205279289616No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCATG150.00222210170.058
CGTATGC150.00222210170.01
CTACTAC150.00222210170.01
GATAAAA207.911986E-570.026
TAAAGAG150.00222210170.064
ATAGACA150.00222210170.04
GTAGGAC150.00222210170.03
AAAAATG301.01978E-770.070
TTATAGA150.00222210170.02
CTTATAG150.00222210170.01
AATCTGG150.00222210170.027
TATAGAC150.00222210170.03
TGAGGCT150.00222210170.013
CAGTAGG150.00222210170.01
TAGGACG150.00222210170.04
CAGTAAA252.8320646E-670.061
TATCGGT207.911986E-570.043
AACAGGT150.00222210170.028
GTTATAC207.911986E-570.01
AGTAAAG150.00222210170.062
Produced by FastQC (version 0.11.5)