FastQCFastQC Report
Wed 25 May 2016
SRR1781275_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781275_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences193473
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT2627613.581223219777435No Hit
CATATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT2027410.478981563318913No Hit
CAAATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT75103.881678580473761No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG61083.157029663053759No Hit
CTAATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT56442.9172029172029172No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG53562.768344937019636No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC37201.9227489107007179No Hit
CACTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC22501.1629529701818857No Hit
TCATATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG20461.0575119008853948No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC8650.44709080853659167No Hit
ATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTT5300.2739400329761775Illumina PCR Primer Index 9 (95% over 22bp)
CATATAAACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT5210.26928822109545003No Hit
CATATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGACGT4850.2506809735725398No Hit
CATATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCAGT4360.2253544422219121No Hit
CATATACACATCTCCGAGCCCACGAGACGTTAACAGATATCGTATGCCGT4070.21036527060623444No Hit
CTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC4030.20829779865924447No Hit
CAAATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGACGT3980.20571345872550692No Hit
CAAATAAACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT3630.18762307918934426No Hit
TCATTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC2970.15350979206400894No Hit
CAAATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCAGT2820.14575677226279637No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGACGT2800.14472303628930136No Hit
CAATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC2790.14420616830255384No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCAGT2670.1380037524615838No Hit
CTTATAAACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT2650.13697001648808876No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAACAGATCTCGTATGCCGT2610.13490254454109876No Hit
CATATACACATCTCCGAGCCCACGAGACGTTAACAGCTCTCGTATGCCGT2540.13128446863386622No Hit
CATATACACATCTCCGAGACCACGAGACGTTAACAGATCTCGTATGCCGT2480.12818326071338118No Hit
CAAATACACATCTCCGAGCCCACGAGACGTTAACAGATATCGTATGCCGT2410.12456518480614867No Hit
CATATACACATCTCCGAGCCCACGAGACGATAACAGATCTCGTATGCCGT2400.12404831681940116No Hit
CTAATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGACGT2380.12301458084590614No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGCTCTCGTATGCCGT2370.12249771285915866No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATATCGTATGCCGT2280.11784590097843109No Hit
AAACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTT2170.11216035312420854Illumina PCR Primer Index 9 (95% over 22bp)
CAAATACACATCTCCGAGACCACGAGACGTTAACAGATCTCGTATGCCGT1980.10233986137600595No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAAAAGATCTCGTATGCCGT1980.10233986137600595No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAAACAGATCTCGTATGCCGT1970.10182299338925845No Hit
CATATACACATCTCCGAGCCAACGAGACGTTAACAGATCTCGTATGCCGT1950.10078925741576344No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGAAGT150.00221650670.0444944
CCGTATA150.00221650670.0444947
AGTCTTA207.887113E-570.0444948
CGACTTA150.00221650670.0444948
CGACTAA150.00221650670.0444948
TAAGCCC150.00221650670.0444943
ACGAATT150.00221650670.0444947
AATCATG150.002218781570.02637554
AGCTTTA207.8972276E-570.02637555
ATGCTAG150.002218781570.02637554
TGAAAAT150.002218781570.02637559
GCATTAA150.002218781570.02637556
AGCATAA150.002218781570.02637555
TTGAATA301.0171243E-770.02637558
ATCTTGA150.002218781570.02637555
ACATGAA150.002218781570.02637556
CAGCTTT150.002218781570.02637554
CCTATGC207.8972276E-570.02637541
TAAGCTT401.3460522E-1070.02637553
TAAGCAT150.002218781570.02637553
Produced by FastQC (version 0.11.5)