FastQCFastQC Report
Wed 25 May 2016
SRR1781274_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781274_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences367825
Sequences flagged as poor quality0
Sequence length76
%GC67

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8471123.030245361245157No Hit
CTTATACACATCTGACGCTGCCGACGATAAACTGGGTGTAGATCTCGGTG25690.6984299598994087No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8700.23652552164752258No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6840.1859579963297764No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4180.11364099775708558No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAT200.006948671352.49928715
CGTATCA4850.051.95805756
GCCGTAT5150.049.61097354
TCTCGGT5450.048.16448243
CCGTATC5250.047.99934855
GTATCAT5250.047.33912757
ATCATTA5350.046.45428559
TAGATCT5350.046.44796839
TATCATT5400.046.02414758
GTAGATC5400.046.0178938
TCATTAA5450.044.9596360
ATCTCGG5700.044.8239542
CATTAAA5550.044.78025461
CGGTGGT5900.043.8977146
TGTAGAT5900.043.30449337
CGCCGTA5950.042.94059453
TCGGTGG6200.042.9026445
AGATCTC5900.042.7112840
GTGTAGA6100.041.88467436
GGTGGTC6100.041.31091347
Produced by FastQC (version 0.11.5)