FastQCFastQC Report
Wed 25 May 2016
SRR1781274_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781274_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences367825
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT228896.222796166655339No Hit
CATATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT179174.871066403860532No Hit
CAAATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT65501.7807381227485894No Hit
TCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG54751.4884795758852716No Hit
CTAATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT48811.3269897369673078No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG42421.1532658193434377No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC32950.895806429688031No Hit
CACTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC19670.5347651736559506No Hit
TCATATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG18150.4934411744715558No Hit
TCTTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC7750.2106980221572759No Hit
CATATAAACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT4790.13022497111398085No Hit
ATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCTT4690.12750628695711275RNA PCR Primer, Index 42 (95% over 22bp)
CATATACACATCTCCGAGCCCACGAGACATCACTCGATATCGTATGCCGT4430.12043770814925576No Hit
CTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC3830.1041256032080473RNA PCR Primer, Index 32 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTAA353.7089194E-969.9902139
CAAATAC12650.064.172021
AGGGGGG45700.063.0898270
AAATACA13700.060.7861562
CATATAC30550.057.2673451
AATACAC24100.057.2041363
ATATACA31000.057.000412
TCGATAT4700.056.58013534
CTCGATA4700.056.58013533
TCGTATC252.3873331E-456.007440
CGATATC4900.055.70649735
GATATCG5100.055.5804636
ATATCGT4850.055.5592437
ATCGTAT4600.055.5357139
TATCGTA4900.054.99231338
AGTCTTC3050.054.00030548
TCGAATG1900.053.42811240
CAGTCTT2850.052.87151747
TATCGGT200.00693346752.52836649
ATCTCGT91500.052.51178437