FastQCFastQC Report
Wed 25 May 2016
SRR1781273_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781273_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences324148
Sequences flagged as poor quality0
Sequence length76
%GC75

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11791936.378135913224824No Hit
CTTATACACATCTGACGCTGCCGACGATAAACTGGGTGTAGATCTCGGTG36211.1170823204215359No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12210.3766797882448758No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11530.3557017164998704No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5960.1838666288238706No Hit
GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4180.12895344102076828No Hit
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4170.1286449399656947No Hit
GGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3730.11507089354245591No Hit
TCTTATACACATCTGACGCTGCCGACGATAAACTGGGTGTAGATCTCGGT3500.10797536927576291No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTGC150.002223459370.023
CGGCAAT150.002223459370.063
TGTAGGT352.9692637E-760.00000437
CTTGTGC650.059.2307661
CTGGTGT252.388468E-455.9999966
GCCGTAT6450.054.2635654
CGCGTCT1100.054.090917
CGTATCA6600.054.09090456
CCGTATC6600.053.030355
TTGTGCG1200.052.52
TAGAACG200.006947556552.54
TACACTG200.006947556552.534
GCGTACT200.006947556552.52
TCTCGGT7050.052.1276643
GTATCAT7000.051.00000457
ATCATTA6950.050.86330859
CGCCGTA6850.050.5839453
TATCATT7000.050.50000458
TCATTAA7000.050.50000460
TCGGTGG7950.049.7484345