FastQCFastQC Report
Wed 25 May 2016
SRR1781273_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781273_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences324148
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT316669.768994409960882No Hit
CATATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT239237.3802707405259325No Hit
CAAATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT91032.8082851043350567No Hit
TCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG77082.377926132507373No Hit
CTAATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT67762.09040314917877No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG60791.8753779137924653No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC41621.283981391216358No Hit
CACTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC26800.8267828275972704No Hit
TCATATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG24040.7416365363969545No Hit
TCTTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC10740.33133013314905535No Hit
CATATAAACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT7120.21965275121240915No Hit
ATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCTT6930.21379123116601056RNA PCR Primer, Index 15 (95% over 21bp)
CATATACACATCTCCGAGCCCACGAGACATAAGTTAATATCGTATGCCGT5480.1690585781803374No Hit
CATATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGACGT5450.16813307501511657No Hit
CATATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCAGT5310.16381406024408604No Hit
CAAATAAACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT4880.15054851487592086No Hit
CAAATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGACGT4640.14314448955415426No Hit
CTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTC4590.14160198427878623No Hit
CAAATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCAGT4130.12741093574540024No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGATAATCTCGTATGCCGT3950.1218579167540753No Hit
TCATTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC3860.11908140725841283No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGACGT3820.1178474030381184No Hit
CTTATAAACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT3810.11753890198304479No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATATCGTATGCCGT3670.11321988721201426No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCAGT3560.10982637560620458No Hit
CAAATACACATCTCCGAGCCCACGAGACATAAGTTAATATCGTATGCCGT3560.10982637560620458No Hit
CAATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTC3370.10396485555980602No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAACGG150.00221683770.0526645
GACGTAT207.890593E-570.0526646
TGACGTA207.890593E-570.0526645
CCGTATA353.6798156E-970.0526647
ACGTATT207.890593E-570.0526647
ACGAATT150.00221683770.0526647
ACACGTA207.9268764E-569.98781638
CGAACAA150.002224995369.98781617
TCGTATC150.002224995369.98781640
TCACGTA1400.067.4882538
ACGTATG1800.066.099640
CAAATAC20900.065.812091
AAATACA21650.065.300412
AGGGGGG66850.065.0148670
AACGTAT650.064.6041339
CACGTAT1300.064.6041339
AATACAC35200.063.2276273
TAACGTA501.4551915E-1162.98903338
AATCACG1950.061.01501536
AAACTCG3200.060.14577536
Produced by FastQC (version 0.11.5)