FastQCFastQC Report
Wed 25 May 2016
SRR1781272_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781272_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences315531
Sequences flagged as poor quality0
Sequence length76
%GC72

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9121228.9074607566293No Hit
CTTATACACATCTGACGCTGCCGACGATAAACTGGGTGTAGATCTCGGTG24840.7872443595082576No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10580.3353077827535171No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7080.2243836580240927No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4140.13120739325137626No Hit
GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3820.1210657589904003No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCATAAG207.91972E-570.02
ATATTCG150.002223404870.048
CGTATCA4300.053.72093256
GCCGTAT4300.053.72093254
CCGTATC4250.053.5294155
CGCCGTA4700.052.8723453
CCGCTAT200.006947387552.524
TAGATCT4700.050.63829839
TTGTGCG900.050.5555532
GTATCAT4650.049.67741857
AGATCTC4800.049.58333240
GTAGATC4950.049.49495338
CTTGTGC1100.047.7272761
TCTCGGT5300.047.54717343
ATCATTA4800.047.39583259
ATCTCGG5200.046.44230742
TATCATT4900.046.4285758
TGTAGAT5600.046.25000437
TGGTCGC5500.045.81818449
CGGTGGT6050.045.7024846
Produced by FastQC (version 0.11.5)