FastQCFastQC Report
Wed 25 May 2016
SRR1781272_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781272_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences315531
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT253108.021398848290659No Hit
CATATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT183315.809571801185937No Hit
CAAATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT68652.1756974750499953No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG61711.9557507820150792No Hit
CTAATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT53801.7050622601265801No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG41851.32633560569326No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC30410.96377218086337No Hit
TCATATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG20000.6338521413109963No Hit
CACTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC18530.5872640089246381No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC8570.27160564255176195No Hit
ATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCTT5530.1752601170724905Illumina PCR Primer Index 9 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTC4750.15053988356136166No Hit
CATATAAACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT4350.1378628407351417No Hit
CATATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGACGT4070.12898891075678776No Hit
CATATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCAGT3630.11504416364794584No Hit
CAAATAAACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT3530.11187490294139087No Hit
CAAATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGACGT3520.11155797687073536No Hit
TCATTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC3250.10300097296303691No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTGCG150.002224522469.991136
GTTATTG150.002224522469.9911325
CAAATAC14550.064.9402161
AGGGGGG49050.063.8553170
AGATAAC550.063.62829635
AATCGTA453.8380676E-1062.21433638
AAATACA15600.061.69092
ACGAATG352.9716102E-759.99239740
AATACAC25950.059.0676543
CACGTAT1550.058.70223239
CGTAATA308.333096E-658.37219248
CATATAC30250.057.3811531
GCCGTAT1350.057.0659846
ATATACA30950.056.874862
CGAAAGA252.3861251E-456.01065441
TAACGTA509.804353E-1055.992938
GTCTACT2450.055.74255849
ACGTATG2100.054.99302740
AACTCGT1600.054.68056537
CCGTCTA2050.054.67053647
Produced by FastQC (version 0.11.5)