FastQCFastQC Report
Wed 25 May 2016
SRR1781271_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781271_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences327730
Sequences flagged as poor quality0
Sequence length76
%GC69

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8545326.07420742684527No Hit
CTTATACACATCTGACGCTGCCGACGATAAACTGGGTGTAGATCTCGGTG22280.6798279071186648No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9980.30451896378116133No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6410.19558783144661765No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4060.12388246422359869No Hit
GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3630.11076190766789736No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTACA207.915811E-570.007782
CGTTATC150.002223846769.997110
GCCGTAT3650.056.57354
CGTATCA3650.055.61413656
CTTGTAC700.055.0061151
TACGCGT407.4620584E-752.505845
GTCATTC200.006948761652.4978342
CTATGCG200.006948761652.4978314
ACGTTAT200.006948761652.497839
TAGCACC200.006948761652.4978346
GTATCAT4000.052.4978257
CCGTATC3950.052.27631855
CATCGTT352.082809E-550.00555832
TATCATT4350.047.469358
TCTCGGT4500.045.8869943
GTCTGGC1150.045.65028410
CGCCGTA4600.045.65028453
TGGTCGC4650.045.15941649
GTAGATC4500.044.33149738
TCGGTGG4900.044.2838845
Produced by FastQC (version 0.11.5)