FastQCFastQC Report
Wed 25 May 2016
SRR1781271_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781271_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences327730
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT183085.586305800506515No Hit
CATATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT131244.004515912488939No Hit
CAAATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT43881.338907027125988No Hit
TCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG40851.2464528727916273No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG40121.2241784395691575No Hit
CTAATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT34981.0673420193451928No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC24450.7460409483416227No Hit
CACTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC15560.4747810697830531No Hit
TCATATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG12360.3771397186708571No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTAGTATGCCGT9180.2801086260031123No Hit
CATATACACATCTCCGAGCCCACGAGACATTGCCGGATCTAGTATGCCGT8760.2672931986696366No Hit
CATATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGACGT8550.2608854850028987No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGACGT7070.21572636011350804No Hit
TCTTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC5810.17728007811308089No Hit
CAAATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGACGT4700.1434107344460379No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCAGGATCTCGTATGCCGT4460.1360876331126232No Hit
CATATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTAAGCCGT4290.13090043633478776No Hit
CAAATACACATCTCCGAGCCCACGAGACATTGCCGGATCTAGTATGCCGT3500.1067952277789644No Hit
CAAATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTAAGCCGT3310.10099777255667775No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGTA301.0149233E-770.07027445
GACGAAA301.0149233E-770.07027446
TACGTAA207.880899E-570.07027446
CAAATAC15300.065.181871
AGGGGGG33700.062.0932970
AAATACA16750.061.419452
ACGTTAA352.9738476E-759.98692327
AATACAC27950.059.843843
ACTTATA3900.058.3206182
CGACATA553.45608E-1157.3302248
CGGAAAT553.6379788E-1157.26024234
CTAATAC12400.056.7215081
TAACGTA252.387477E-456.00488338
ATATACA30050.055.8946342
GTATTCT2950.055.7931249
TAATACA12850.055.0074652
CATATAC30250.054.8310241
CGGATAT4050.054.4325834
CGGATAA452.7002898E-854.43257534
CCGTAAT3800.054.39666447
Produced by FastQC (version 0.11.5)