FastQCFastQC Report
Wed 25 May 2016
SRR1781270_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781270_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences448307
Sequences flagged as poor quality0
Sequence length76
%GC67

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10765824.014347311106004No Hit
CTTATACACATCTGACGCTGCCGACGATAAACTGGGTGTAGATCTCGGTG50141.1184300044389224No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11250.2509441074977638No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10380.23153776318460342No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5690.12692195303664675No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTATC207.924227E-569.99843655
CGTATCA8100.057.03576356
GCCGTAT8100.057.03576354
GTATCAT8650.055.0338157
ATCTCGG8650.054.6230642
CCGTATC8350.054.48980355
TCTCGGT8750.054.39878543
CGCCGTA8550.053.6245453
TGGTCGC8800.052.89654549
GTAGATC9350.052.77957538
GACGTAT200.00694989952.49882554
GATCTCG9300.052.31066541
TAGATCT9200.052.11840439
AGATCTC9300.051.93432640
TCATTAA8800.051.70915660
ATCATTA8900.051.12815559
CATTAAA9050.050.667561
TCGCCGT9000.050.1655552
TATCATT9000.049.78222358
TCGGTGG9950.049.59688645