FastQCFastQC Report
Wed 25 May 2016
SRR1781270_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781270_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences448307
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT292746.529900269235145No Hit
CATATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT211424.715964729526864No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG80421.7938600111084593No Hit
CAAATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT75041.6738529623673064No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG70531.573252257939314No Hit
CTAATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT58301.3004481304106337No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC40070.8938071455498129No Hit
CACTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC23810.5311092621797117No Hit
TCATATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG22080.4925196349822778No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC9450.21079305029812162No Hit
CATATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGACGT8240.18380261740280657No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGACGT7050.15725830736526533No Hit
CAAATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGACGT5840.13026787446995028No Hit
CATATAAACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT5630.12558358446332535No Hit
CTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTC5500.12268378588779563Illumina PCR Primer Index 10 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCTT5490.12246072445890874Illumina PCR Primer Index 10 (95% over 23bp)
CATATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCAGT4810.10729254729459946No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATA207.884893E-570.0687947
CGAATTA207.884893E-570.0687948
TACCGTC353.6779966E-970.0687945
TCGTAAT252.840423E-669.9906340
CAAATAC17650.063.4405171
AGGGGGG52800.062.3615970
CCGAAAT401.0570147E-861.3101947
TAAGCCG4500.059.9276643
TAACTCG2200.058.84919436
AAATACA19850.058.5246772
AATACAC31400.057.390373
CGACTTA553.45608E-1157.32900648
GCCGTAT3250.057.1330146
ATATACA39300.056.538292
AAGCCGT4300.056.2054344
CATATAC39000.056.1657031
ATACCGT509.731593E-1056.04251544
TCGTATA509.804353E-1055.99250440
CTCGTAA5300.055.45808839
TGCCGTA4750.055.31746345
Produced by FastQC (version 0.11.5)