FastQCFastQC Report
Wed 25 May 2016
SRR1781269_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781269_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences319240
Sequences flagged as poor quality0
Sequence length76
%GC73

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9980831.26425259992482No Hit
CTTATACACATCTGACGCTGCCGACGATAAACTGGGTGTAGATCTCGGTG30250.9475629620348327No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12050.3774589650419747No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7550.23649918556571856No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5170.1619471244204987No Hit
GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5150.16132063651171533No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGCGCG150.00222342870.0000153
TACACGT252.3884122E-456.0000045
GCCGTAT5250.053.33333254
GTTCTAG200.0069474652.5000041
GTATTCT200.0069474652.50000436
TAGACAT200.0069474652.5000045
ACGGATT200.0069474652.50000448
GGACCTT200.0069474652.5000046
CTTTAGG200.0069474652.50000418
ATACGCG200.0069474652.5000044
CGATAGG200.0069474652.50000425
CCGTATC5500.050.27272855
TCGGTGG6350.050.15748245
CGTATCA5550.049.8198256
TGGTCGC6050.049.75206849
ATCATTA5650.049.5575259
GTATCAT5650.048.93805357
TATCATT5600.048.75000458
TAGATCT6050.048.59504339
TCTCGGT6200.048.54838643
Produced by FastQC (version 0.11.5)