FastQCFastQC Report
Wed 25 May 2016
SRR1781269_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781269_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences319240
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT289669.07342438290941No Hit
CATATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT194516.092908156872572No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG64972.0351459716827462No Hit
CAAATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT64182.010399699285804No Hit
CTAATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT53061.6620724220022551No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG51851.6241699035208619No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC40881.2805412855531888No Hit
CACTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC23400.7329908532765318No Hit
TCATATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG20210.6330660318255857No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC9440.2957022929457462No Hit
CATATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGACGT5740.17980202982082447No Hit
ATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCTT5590.17510337050494926RNA PCR Primer, Index 24 (95% over 23bp)
CATATAAACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT5380.168525247462724No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGACGT4770.14941736624483148No Hit
CTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC4530.14189951133943116No Hit
CATATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCAGT4250.1331286806164641No Hit
CAAATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGACGT3990.12498433780228041No Hit
CTTATAAACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT3700.11590026312492169No Hit
CAAATAAACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT3260.10211752913168776No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGAATCTCGTATGCCGT3230.10117779726851273No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGTC207.870707E-570.0880145
ATTCCGT252.8178201E-670.0770244
AACGTAT401.3460522E-1069.989239
TCGTATT401.3460522E-1069.989240
ATCGTAA301.0230178E-769.9891939
ACGTTAA150.002226173469.97822627
TAACGTA401.3460522E-1069.97822638
CGTTATA301.0241274E-769.97822628
CGGATTT150.002226173469.97822612
CAAATAC15700.065.531281
AGGGGGG53600.064.9619870
AAATACA16350.063.9862062
TCGAAAG550.063.62653440
CCGAATT401.0521035E-861.3366447
TCGTATC401.0652002E-861.24054740
AATACAC28050.060.248643
CGACTTA352.9402327E-760.0848648
CTAATAC11750.058.6717871
TAAGCCG3450.057.88971343
ATCGTAT3000.057.15783339
Produced by FastQC (version 0.11.5)