FastQCFastQC Report
Wed 25 May 2016
SRR1781268_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781268_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences173251
Sequences flagged as poor quality0
Sequence length76
%GC90

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10662161.54134752468961No Hit
CTTATACACATCTGACGCTGCCGACGATAAACTGGGTGTAGATCTCGGTG33911.9572758598795967No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10990.6343397729306035No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9350.5396794246497856No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5150.2972565814915931No Hit
GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4170.24069125142134823No Hit
TCTTATACACATCTGACGCTGCCGACGATAAACTGGGTGTAGATCTCGGT3250.18758910482479177No Hit
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3070.17719955440372637No Hit
GGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2970.17142758194757896No Hit
GGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2610.15064848110544815No Hit
GGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2110.12178861882471095No Hit
GGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2020.11659384361417827No Hit
CTCTTATACACATCTGACGCTGCCGACGATAAACTGGGTGTAGATCTCGG1980.1142850546317193No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1880.10851308217557185No Hit
GGGGGGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGG1830.10562709594749814No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGTA150.00222170870.059
TGCATGG150.00222170870.046
AGAGGAG150.00222170870.066
ATGGTCA150.00222170870.049
GATAAAA150.00222170870.026
GGGAGTA150.00222170870.034
AGGGACC150.00222170870.049
ACCCCCC150.00222170870.053
CATGGTC150.00222170870.048
CTGCCCA150.00222170870.04
GGTATAC150.00222170870.01
CCGACAC150.00222170870.024
GTTGTGC150.00222170870.01
CGCGTCT550.063.6363687
GTTGTGG501.4551915E-1162.9999961
TTGTGGG650.059.2307662
TACACGT308.350216E-658.3333325
TTGTGCG601.8189894E-1258.3333322
ATACACG252.3853542E-455.9999964
TGTGTAG252.3853542E-455.99999635
Produced by FastQC (version 0.11.5)