FastQCFastQC Report
Wed 25 May 2016
SRR1781268_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781268_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences173251
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT2690015.526605907036611No Hit
CATATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT2301813.285926199560175No Hit
CAAATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT87915.074140986199214No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG69243.996513728636487No Hit
CTAATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT60593.4972381111797333No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG54473.14399339686351No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC33131.9122544747216468No Hit
TCATATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG24781.4302947746333354No Hit
CACTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC24651.4227912104403437No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC9810.5662304979480638No Hit
CATATAAACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT7850.45309983780757396No Hit
CATATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGACGT5560.32092166856179766No Hit
ATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCTT5540.3197672740705681TruSeq Adapter, Index 12 (95% over 23bp)
CAAATAAACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT5330.3076461319126585No Hit
CTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTC4760.2747458889126181TruSeq Adapter, Index 12 (95% over 21bp)
CAAATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGACGT4710.27185990268454435No Hit
CATATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCAGT4260.24588602663188092No Hit
CTTATAAACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT4110.23722806794765974No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGACGT3850.22222093956167638No Hit
TCATTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC3480.20086464147393088No Hit
CATATACACATCTCCGAGCCCACGAGACTACTTGAAATATCGTATGCCGT3230.1864347103335623No Hit
CAATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTC3140.18123993512302958TruSeq Adapter, Index 12 (95% over 21bp)
CAAATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCAGT3120.1800855406318001No Hit
CTAATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGACGT3080.17777675164934115No Hit
AAACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCTT2930.16911879296511997RNA PCR Primer, Index 12 (95% over 23bp)
CTAATAAACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT2860.16507841224581676No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCAGT2660.15353446733352188No Hit
CATATACACATCTCCGAGACCACGAGACTACTTGAAATCTCGTATGCCGT2320.13390976098262058No Hit
CAAATACACATCTCCGAGCCCACGAGACTACTTGAAATATCGTATGCCGT2290.13217816924577636No Hit
CATATACACATCTCCGAGCCAACGAGACTACTTGAAATCTCGTATGCCGT2230.1287149857720879No Hit
CAAATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGA2200.12698339403524367No Hit
TTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTC2200.12698339403524367TruSeq Adapter, Index 12 (95% over 21bp)
CATATACACATCTCCGAGCCCACGAGACTACTAGAAATCTCGTATGCCGT2160.12467460505278469No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC2150.12409740780716995No Hit
CTTATACACATCTCCGAGCCCACGAGACTACATGAAATCTCGTATGCCGT2140.1235202105615552No Hit
CCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG2140.1235202105615552No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTAGAAATCTCGTATGCCGT2140.1235202105615552No Hit
CAAATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTAAGCCGT2020.11659384361417827No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATATCGTATGCCGT1930.11139906840364558No Hit
CAAATACACATCTCCGAGACCACGAGACTACTTGAAATCTCGTATGCCGT1880.10851308217557185No Hit
CATATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGA1830.10562709594749814No Hit
CAAATACACATCTCCGAGCCAACGAGACTACTTGAAATCTCGTATGCCGT1810.10447270145626864No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCAG1780.10274110971942442No Hit
CTAATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCAGT1770.10216391247380968No Hit
CATATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTAAGCCGT1770.10216391247380968No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTATT150.002215679370.0479547
GAAGTAT150.002215679370.0479546
GTATTAC150.002215679370.0479542
TATGATA150.002215679370.0479553
TAAGACG401.3278623E-1070.0479543
GAATGCA207.88286E-570.0479542
TCAGCAA150.002215679370.0479553
GACGTAA252.8193153E-670.0479546
TCTGATA150.002215679370.0479553
CGTAAGA401.3278623E-1070.0479541
AAAGCCG207.88286E-570.0479543
ACGAATT207.88286E-570.0479547
AATGCAG207.88286E-570.0479543
CCGACAT850.070.0479547
CGACTAA207.88286E-570.0479548
TGACGAA252.8193153E-670.0479545
AGAAACA150.002218219770.02772559
TTGATAA207.894149E-570.0277258
CAACAGC150.00222076270.0075151
TTAAGCT301.0183976E-770.0075152
Produced by FastQC (version 0.11.5)