FastQCFastQC Report
Wed 25 May 2016
SRR1781267_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781267_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences246069
Sequences flagged as poor quality0
Sequence length76
%GC86

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11748947.74636382478085No Hit
CTTATACACATCTGACGCTGCCGACGATAAACTGGGTGTAGATCTCGGTG34151.3878221149352417No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11590.4710060999150644No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11290.4588143975876685No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5950.24180209616002016No Hit
GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4490.18246914483335974No Hit
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3660.1487387683942309No Hit
TCTTATACACATCTGACGCTGCCGACGATAAACTGGGTGTAGATCTCGGT3580.14548764777359197No Hit
GGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3440.13979818668747385No Hit
GGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3230.13126399505829664No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2870.11663395226542148No Hit
GGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2710.11013171102414362No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGTGC800.070.01
TATCAAT207.9162586E-570.058
ACTATCT150.00222282170.039
TATGGGC150.00222282170.03
CTTATGC150.00222282170.01
TATCTCT150.00222282170.041
GTAGATA150.00222282170.038
TAGATGT150.00222282170.039
TAGATAT150.00222282170.039
ATGAAAA150.00222282170.062
TATAGAC150.00222282170.03
TCTTTAT150.00222282170.01
CTTTATA150.00222282170.02
AGTAGAT207.9162586E-570.037
TTGTGCG750.065.3333362
CTTGTGG401.0624717E-861.251
CTTGGGG401.0624717E-861.251
TTGTGGG650.059.2307662
ATCAATA308.360614E-658.33333259
TCAATAA308.360614E-658.33333260
Produced by FastQC (version 0.11.5)