FastQCFastQC Report
Wed 25 May 2016
SRR1781267_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781267_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences246069
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT3201313.009765553564245TruSeq Adapter, Index 5 (95% over 23bp)
CATATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT214068.699186000674608TruSeq Adapter, Index 5 (95% over 23bp)
TCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG73332.9800584388931557TruSeq Adapter, Index 5 (95% over 22bp)
CAAATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT72002.9260085585750337TruSeq Adapter, Index 5 (95% over 23bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG59572.4208656921432605No Hit
CTAATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT59202.4058292592728057TruSeq Adapter, Index 5 (95% over 23bp)
CTCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC38211.5528164864326672RNA PCR Primer, Index 5 (95% over 21bp)
TCATATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG21950.8920262202878054TruSeq Adapter, Index 5 (95% over 22bp)
CACTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC21410.8700811560984926TruSeq Adapter, Index 5 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC10220.4153306592866228RNA PCR Primer, Index 5 (95% over 21bp)
CATATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGACGT7510.30519894826247923No Hit
ATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTCTT5940.2413957060824403TruSeq Adapter, Index 5 (96% over 26bp)
CATATAAACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT5550.22554649305682553RNA PCR Primer, Index 5 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGACGT5490.2231081525913463No Hit
CATATACACATCTCCGAGCCCACGAGACACAAAGTGATATCGTATGCCGT5160.20969728003121077No Hit
CTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTC4950.20116308840203356TruSeq Adapter, Index 5 (95% over 24bp)
CATATACACATCTCCGAGCCCACGAGACACAAAGTGATCACGTATGCCGT4860.19750557770381477No Hit
CAAATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGACGT4780.19425445708317587No Hit
CATATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCAGT4340.17637329366966176No Hit
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATATCGTATGCCGT3980.1617432508767866No Hit
CAAATACACATCTCCGAGCCCACGAGACACAAAGTGATCACGTATGCCGT3600.1463004279287517No Hit
CTAATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGACGT3580.14548764777359197No Hit
CAAATAAACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT3350.13614067598925506No Hit
CATATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTAAGCCGT3290.13370233552377586No Hit
TCATTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC3280.133295945446196No Hit
CATATACACATCTCCGCGCCCACGAGACACAAAGTGATCTCGTATGCCGT3160.1284192645152376TruSeq Adapter, Index 5 (95% over 23bp)
CAAATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTAAGCCGT3120.12679370420491815No Hit
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCAGT3090.12557453397217855No Hit
CTTATAAACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT3060.12435536373943894No Hit
CTTATACACATCTCCGAGCCCACGAGACAAAAAGTGATCTCGTATGCCGT3040.12354258358427919No Hit
CTTATACACATCTCCGCGCCCACGAGACACAAAGTGATCTCGTATGCCGT3040.12354258358427919TruSeq Adapter, Index 5 (95% over 23bp)
CAAATACACATCTCCGAGCCCACGAGACACAAAGTGATATCGTATGCCGT3020.12272980342911949No Hit
CAATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTC3010.12232341335153961TruSeq Adapter, Index 5 (95% over 24bp)
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCACGTATGCCGT2910.11825951257574095No Hit
CATATACACATCTCCGAGCCCACGAGACACAAAGTGATCTAGTATGCCGT2830.11500839195510203No Hit
CAAATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCAGT2730.11094449117930337No Hit
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTAGTATGCCGT2540.10322307970528591No Hit
TTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTC2490.1011911293173866TruSeq Adapter, Index 5 (95% over 24bp)
CTTATACACATCTCCGAGCCCACGAGACACAAAGAGATCTCGTATGCCGT2480.10078473923980673No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGAATT150.002211192470.0926147
GCAGTAT301.01163096E-770.0926146
AGTCTAA150.002211192470.0926148
TGCCTAC150.002211192470.0926145
CCGAATT301.01163096E-770.0926147
CGACTAA207.864567E-570.0926148
AGAATTC207.864567E-570.0926148
CAGTATT252.8121758E-670.09260647
CTAAGCC207.872502E-570.0783542
ATATGAC150.002212978270.0783542
ATTCAGT150.002212978270.0783544
AAACCGT150.002212978270.0783544
ATGATAG207.880443E-570.0640954
ACTGATT252.8189843E-670.0640953
CAGCATA207.880443E-570.0640954
CGAAAGA150.002216553570.04983541
ATTAAGC401.3460522E-1070.0355951
TACTGAT353.6834535E-970.0355952
TATTAAG353.6834535E-970.0355950
TAATTCT150.002218342670.0355852
Produced by FastQC (version 0.11.5)