FastQCFastQC Report
Wed 25 May 2016
SRR1781266_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781266_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences696637
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8705012.495747426565055No Hit
CTTATACACATCTGACGCTGCCGACGATTGGTACGGTGTAGATCTCGGTG15890.22809583757394453No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9780.14038875339667575No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTTCGG200.00695089452.549
GCCGTAT2900.049.48275854
TTGTGCG551.3116187E-744.5454522
TGTGCGC950.044.2105263
TCTCGGT3550.041.4084543
CGCCGTA3450.040.57971253
CCGTATC3500.040.055
CGGTGGT3950.039.8734246
CGGTAAG459.1981645E-538.8888928
CGTATCA3850.038.18181656
TGGTCGC4350.036.20689449
GTATCAT4000.035.87500457
CTCGGTG4100.035.8536644
GTAGATC4300.035.81395338
GTGGTCG4450.035.39325748
TTCGGTA501.7097931E-435.00000426
TCGGTGG4550.034.61538345
ATCTCGG4300.034.18604742
AGATCTC4550.033.84615340
GATCTCG4500.032.66666441
Produced by FastQC (version 0.11.5)