FastQCFastQC Report
Wed 25 May 2016
SRR1781266_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781266_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences696637
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT422786.068870875362635No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG78291.1238277610864769No Hit
CTTAAACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT57770.8292697631621634No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC45180.6485443638509009No Hit
CATATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT30840.4426982775821554No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28380.40738576905906515No Hit
CATAAACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT11490.16493525322370187No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC10700.15359505739718102No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG48850.061.96386770
GCCGTAT950.051.62119346
ACGTATG1600.050.31033340
TATGCCG87950.050.1361643
ATGCCGT87500.050.08098644
CGTATGC91300.049.73283841
GTATGCC89000.049.62337542
TCGTATG94050.049.4928240
CCGTCTT83700.049.46700747
CTCGTAT90500.049.46195639
TGCCGTC87850.049.3631145
TCTCGTA91200.049.27419738
GCCGTCT85650.049.19963546
CGTCTTC86800.049.0724248
GCTTGAA90400.049.05561456
GCCGTCA4000.049.0401346
CGACAAT2000.048.99085632
AATCTCG92800.048.90789436
CTTGAAA91450.048.7950557
TAAACAC19600.048.7409023