FastQCFastQC Report
Wed 25 May 2016
SRR1781265_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781265_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences148203
Sequences flagged as poor quality0
Sequence length76
%GC79

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5497137.091691801110635No Hit
CTTATACACATCTGACGCTGCCGACGATTGGTACGGTGTAGATCTCGGTG9400.6342651633232795No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6530.4406118634575548No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4160.2806960722792386No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2880.19432805003947287No Hit
GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2330.15721679048332354No Hit
GGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1800.12145503127467056No Hit
GGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1780.1201055309271742No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTGCG150.002221071970.02
AAGCAAG150.002221071970.062
TGGGGAA150.002221071970.04
TATCATT1800.056.38888558
CGTATCA1750.056.00000456
GTATCAT1750.056.00000457
TCATTAA1900.055.26315760
CCGTATC1850.054.86486455
GCCGTAT1850.054.86486454
AAGGTCG200.00694015452.55
GGTCACT200.00694015452.540
TGTGCAA200.00694015452.536
CTTGGTA200.00694015452.570
CAAATAA200.00694015452.550
AGCGAGT200.00694015452.515
GCAATTT200.00694015452.513
CATCCCT200.00694015452.541
CGCCGTA2000.050.74999653
CATTAAA2100.050.00000461
GTAGATC2100.050.00000438