FastQCFastQC Report
Wed 25 May 2016
SRR1781265_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781265_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences148203
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT2535317.106941155037347No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG48833.294805098412313No Hit
CTTAAACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT37112.503997894779458No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC31252.1085942929630304No Hit
CATATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT20891.4095531129599266No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18031.21657456326795No Hit
CATAAACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT8230.5553193929947438No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC6950.46895137075497795No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGACGT5180.3495205900015519No Hit
ATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCTT4420.298239576796691TruSeq Adapter, Index 2 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGCGCACGTATCTCGTATGCCGT3420.23076455942187407No Hit
CTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTC3410.2300898092481259No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCAGT3240.218619056294407No Hit
CTTATACACATCTCCGCGCCCACGAGACGAGCACGTATCTCGTATGCCGT3080.20782305351443628No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGCACGAATCTCGTATGCCGT2950.1990513012557101No Hit
CTCTAATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC2460.16598854274204974No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGCAAGTATCTCGTATGCCGT2370.15991579117831622No Hit
TCTTAAACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG2370.15991579117831622No Hit
CTTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG2240.15114403891959002No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGAACGTATCTCGTATGCCGT2170.14642078770335284No Hit
CACTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC2040.13764903544462664No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC2000.13495003474963393No Hit
CTTAAACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGACGT1980.13360053440213762No Hit
ATACCCATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTCTT1830.12347928179591507TruSeq Adapter, Index 2 (95% over 21bp)
TTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGTC1740.11740653023218153No Hit
CCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG1600.10796002779970715No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATGACG207.8858815E-570.0357943
CGAATTA150.002216571770.0357948
AAGTCTT150.002216571770.0357947
ACGAATT150.002216571770.0357947
CGTTGCT150.002219542470.0121540
TCAACTG301.016906E-770.0121550
ACGAAAG150.002219542470.0121540
CCGGAAA150.00222251669.988534
CTTATAG150.00222251669.988535
GCGTATC150.00222251669.9885333
CGAATAT207.9123056E-569.98852534
AGGGGGG29900.064.83887570
GTATCTA1200.061.23996435
TAACTCG1200.061.23996436
TCTAGTA1150.060.8595938
CTTAAAC7950.060.7447621
CATAAAC3550.060.130991
TAAACAC11300.059.7689633
TTAAACA8100.059.6198582
ATATACA5250.059.323612
Produced by FastQC (version 0.11.5)