FastQCFastQC Report
Wed 25 May 2016
SRR1781264_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781264_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences328602
Sequences flagged as poor quality0
Sequence length76
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6507919.804809465554072No Hit
CTTATACACATCTGACGCTGCCGACGATTGGTACGGTGTAGATCTCGGTG9000.2738875600270236No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8740.265975252737354No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6180.18806945788522286No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3520.10712046792168033No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAATCG252.3885164E-456.0000045
TAACGTT200.00694764152.50000412
ATAACGT200.00694764152.50000411
CGTATCA2200.042.95454456
TTGGGCG503.4732784E-642.0000042
GCCGTAT2100.041.66666454
TCGAAAC556.6846223E-638.18181624
ATCGAAA556.6846223E-638.18181623
CCGTATC2250.037.33333255
GGTCAAC501.7078314E-435.0000041
TGTGCGC806.228038E-835.0000043
ATTCAAC400.002416927835.00000444
TGCGCGT904.489266E-934.9999965
GTAGATC2550.034.31372538
CGGTGGT3000.033.83333246
TGGTCGC2600.033.65384749
TCGGTGG3400.032.94117745
TTGTGCG751.4875459E-632.6666682
CTTGTGG652.0994785E-532.3076931
CTTGTGC652.0994785E-532.3076931