FastQCFastQC Report
Wed 25 May 2016
SRR1781264_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781264_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences328602
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT309459.417167272262494No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG56361.71514476479145No Hit
CTTAAACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT42151.2827067394598939No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC31080.9458250406266547No Hit
CATATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT26440.804620787457167No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18650.5675558882782211No Hit
CATAAACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT9770.2973201623848911No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC8620.2623234186036604No Hit
ATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTCTT5940.18076578961783554RNA PCR Primer, Index 47 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGACGT5340.16250661894936733No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGAAGATCTCGTATGCCGT4250.12933579223498337No Hit
CTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGTC4160.12659691663471312No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCAGT3790.11533709472249104No Hit
CTTATACACATCTCCGAGCCCACGAGACAAAGGTAGATCTCGTATGCCGT3690.11229389961107966No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTTA150.002224368769.99300470
AACGTAT150.002224368769.99300439
TCAGTCG150.002225711769.9823566
TCGAAAG453.8380676E-1062.21600340
AGGGGGG37750.062.02028370
ACGTATC252.3896963E-455.99440440
AACACAT15350.054.709335
TAAACAC14950.053.8325773
CTTAAAC10900.053.289311
TAAGCCG1450.053.16286543
GGTAACG200.00692949652.5347252
GTTACGC200.00695457552.4867621
CGATATA200.00695457552.4867629
TATGCCG67950.050.8957343
CCGTCTT64400.050.8331547
CGAATGC2000.050.76037641
CGTCTTC66800.050.73831648
GTATGCC68250.050.7233542
CTCGTAA1450.050.6845939
ATGCCGT67450.050.65735644
Produced by FastQC (version 0.11.5)