FastQCFastQC Report
Wed 25 May 2016
SRR1781263_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781263_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences389757
Sequences flagged as poor quality0
Sequence length76
%GC70

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7595019.48650056317141No Hit
CTTATACACATCTGACGCTGCCGACGATTGGTACGGTGTAGATCTCGGTG13550.3476525116931832No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9330.2393799213356014No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6540.16779685804231867No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4250.10904230071557407No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGTAT207.922054E-570.054
ATACGAA150.002223798570.053
TATACGA150.002223798570.052
CACTTGA150.002223798570.010
GCCGTAT2450.055.71428754
TAGATCT2750.053.45454439
ACTTGCA200.006948607552.545
CGTTAGT200.006948607552.514
TCAATCG200.006948607552.568
TACGAAT200.006948607552.554
TATCGAG200.006948607552.528
TCATTAA2950.051.0169560
CGTATCA2850.050.3508856
ATCATTA3000.050.16666859
TATCATT2950.049.8305158
CCGTATC2700.049.25926255
CATTAAA3150.048.8888961
TCTCGGT3050.047.0491843
TTGTGAT305.8745965E-446.66666437
ACTACTT305.8745965E-446.6666643
Produced by FastQC (version 0.11.5)