FastQCFastQC Report
Wed 25 May 2016
SRR1781263_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781263_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences389757
Sequences flagged as poor quality0
Sequence length76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT369319.47539107700439No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG63851.6382002119269186No Hit
CTTAAACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT51221.3141521512121657No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC40031.0270501876810423No Hit
CATATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT28830.7396916540305883No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25970.6663125999019902No Hit
CATAAACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT10580.2714511862519467No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC10340.26529350338800844No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGACGT5900.15137637040514987No Hit
ATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTT5350.13726501384195794Illumina PCR Primer Index 9 (95% over 22bp)
CTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTC4810.12341022739809676No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCAGT4660.11956167560813531No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAACAGATCTCGTATGCCGT4430.11366056286352778No Hit
CTTATACACATCTCCGCGCCCACGAGACGTTAACAGATCTCGTATGCCGT4410.11314742262486625No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAA207.875618E-570.08311546
AGGGGGG46700.064.5143670
CGTTAAA1350.059.61618828
ATATCGT2300.059.33444637
TAAACAC17100.058.9340863
CCGTAAT308.311066E-658.40259647
CGTAAAA308.380939E-658.32018328
AAACACA19700.058.083354
AACACAT18350.057.3985065
CTTAAAC11650.056.7683141
CATAAAC6250.056.547251
ATCGTAT2600.056.5257239
TTAAACA12000.055.1125762
AAAGATC1600.054.6751733
GATATCG2850.054.0229136
TTAAAAG1700.053.5173530
ACGTATG1700.053.5173540
CGTCTTA1050.053.3966648
AGTTAAC1250.053.1880128
AAAGCCG608.367351E-1152.54883643
Produced by FastQC (version 0.11.5)