FastQCFastQC Report
Wed 25 May 2016
SRR1781262_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781262_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences128193
Sequences flagged as poor quality0
Sequence length76
%GC89

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7727460.27942243336219No Hit
CTTATACACATCTGACGCTGCCGACGATTGGTACGGTGTAGATCTCGGTG14621.1404678882622297No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8510.6638427995288354No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5740.44776235831909694No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3870.301888558657649No Hit
GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2940.22934169572441554No Hit
GGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2130.1661557183309541No Hit
GGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1820.14197343068654295No Hit
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1670.13027232376182787No Hit
GGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1470.11467084786220777No Hit
TCTTATACACATCTGACGCTGCCGACGATTGGTACGGTGTAGATCTCGGT1440.11233062647726474No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATTAA207.901807E-570.0000160
AAGGTGT207.901807E-570.0000133
TTGTGCG252.826604E-670.000012
GTGTGCG252.826604E-670.000012
AACATTA207.901807E-570.0000159
GTAGATA150.002220384370.038
ACCCGTG150.002220384370.052
CCGTAAC150.002220384370.055
TCTCGGA150.002220384370.039
TAACATT150.002220384370.058
CTTGTGC150.002220384370.01
CGTAACA150.002220384370.056
TACCCGT150.002220384370.051
GCCGTAA150.002220384370.054
TCATCAT150.002220384370.02
GGGTGTA150.002220384370.034
GTGTGGC150.002220384370.010
TAGATAT150.002220384370.039
AGATATC150.002220384370.040
TTGTGGG600.064.1666642
Produced by FastQC (version 0.11.5)