FastQCFastQC Report
Wed 25 May 2016
SRR1781262_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781262_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences128193
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT3732429.115474323871037No Hit
TCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG70935.53306342780027No Hit
CTTAAACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT53584.179635393508225No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC41523.2388663967611335No Hit
CATATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT27332.131941681683087No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24941.945504044682627No Hit
TCTTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC10660.8315586654497515No Hit
CATAAACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT10390.8104966729852644No Hit
ATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCTT5660.44152176795924897RNA PCR Primer, Index 42 (95% over 22bp)
CTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC5110.39861770923529366RNA PCR Primer, Index 32 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGACGT4710.36741475743605345No Hit
CTTATACACATCTCCGCGCCCACGAGACATCACTCGATCTCGTATGCCGT4590.3580538718962814No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATATCGTATGCCGT4430.3455726911765853No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCAGT4290.3346516580468512No Hit
CTTATACACATCTCCGAGCCCACGAGACATAACTCGATCTCGTATGCCGT3440.26834538547346576No Hit
TCTTAATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC3310.2582044261387127No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGCTCTCGTATGCCGT3150.24572324541901666No Hit
TTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC3050.2379225074692066RNA PCR Primer, Index 32 (95% over 21bp)
CTTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG3000.2340221384943016No Hit
CTCTAATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC2960.23090184331437755No Hit
TCTTAAACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG2930.22856162192943452No Hit
CCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG2490.1942383749502703No Hit
TCTTATACACATCTCCGAGCCCACGAGACATCACTCGCTCTCGTATGCCG2450.19111807977034628No Hit
ATACCCATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCTT2420.18877785838540326RNA PCR Primer, Index 42 (95% over 22bp)
CTTTACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC2370.18487748941049825No Hit
CACTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC2260.17629667766570717No Hit
CTTATACACATCTCCGAGCCCACGAGACATCAATCGATCTCGTATGCCGT2160.16849593971589713No Hit
TCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCAG2110.1645955707409921No Hit
TATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCT2050.15991512797110605RNA PCR Primer, Index 42 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGACG1880.14665387345642897No Hit
CTTAAACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCAGT1820.14197343068654295No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACACGATCTCGTATGCCGT1760.13729298791665692No Hit
CTAATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT1750.1365129141216759No Hit
CTTATACACATCTCCGAGCCAACGAGACATCACTCGATCTCGTATGCCGT1710.1333926189417519No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTAGATCTCGTATGCCGT1710.1333926189417519No Hit
CTTAAACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGACGT1690.13183247135178988No Hit
CTTAAACACATCTCCGAGCCCACGAGACATCACTCGATATCGTATGCCGT1580.12325165960699883No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATCACTCGCTCTCGTATGCC1480.11545092165718877No Hit
CTTATACACATCACCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT1370.1068701099123977No Hit
CTTAATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG1370.1068701099123977No Hit
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGA1340.10452988852745469No Hit
CTTATACACATCTCCGAGACCACGAGACATCACTCGATCTCGTATGCCGT1340.10452988852745469No Hit
CATATACACATCTCCGAGCCCACGAGACATCACTCGATATCGTATGCCGT1300.10140959334753068No Hit
CATATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGACGT1290.10062951955254967No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCAGT150.002208519570.09457444
CGAATTC150.002208519570.09457448
GCAGACA150.002208519570.09457446
TGCAGTA252.8040067E-670.09457445
CGACTTA150.002208519570.09457448
GCAGTAT207.849104E-570.0945746
AGTCTTA207.849104E-570.0945748
CGAATGA700.070.0671941
AATCTGC207.894817E-570.0124951
AATTCTG150.002218811370.0124950
CACGTAA150.002218811370.0124939
CATCTGA252.8236045E-670.0124951
ACGTAAG150.002218811370.0124940
ACTTCAG301.01577825E-770.0124950
TCTAATG252.8236045E-670.0124950
TCACGAA150.002218811370.0124938
CTAATGC207.894817E-570.0124951
ACGAATG150.002218811370.0124940
GACTTAT150.002218811370.0124949
CTTCTGT150.002218811370.0124950
Produced by FastQC (version 0.11.5)