FastQCFastQC Report
Wed 25 May 2016
SRR1781261_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781261_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences286312
Sequences flagged as poor quality0
Sequence length76
%GC78

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10489436.63625695045964No Hit
CTTATACACATCTGACGCTGCCGACGATTGGTACGGTGTAGATCTCGGTG19650.686314230629523No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10590.3698762189499567No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10150.3545083684931124No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5260.1837156668250021No Hit
GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3760.13132526754030568No Hit
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3340.11665595574059068No Hit
GGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3090.10792422252647461No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACAG150.002223193970.035
CCGCAAA150.002223193970.030
CGCCAAA308.364097E-658.33333220
GCCGTAT3700.052.97297354
CTTGTAC200.00694673252.5000041
GCGAATG200.00694673252.5000048
TTGTACG200.00694673252.5000042
GGATACA200.00694673252.50000454
CTAACTT200.00694673252.50000460
ACCTACG200.00694673252.50000415
AGTTGAA200.00694673252.50000468
TGCGACA200.00694673252.50000451
AATTCAT200.00694673252.50000466
TTTGCAT200.00694673252.50000450
GTATCAT3850.051.8181857
CCGTATC3750.051.33333655
CGTATCA3900.051.15384756
TATCATT3750.050.458
AGATCTC4050.048.3950640
CGCCGTA4000.048.12499653