FastQCFastQC Report
Wed 25 May 2016
SRR1781261_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781261_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences286312
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT5087817.770124898711895No Hit
TCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG88313.08439744055436No Hit
CTTAAACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT73452.565383218307301No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC51811.8095643912934143No Hit
CATATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT39781.3893933890301489No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG35021.223141188633379No Hit
CATAAACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT15470.5403196512895023No Hit
TCTTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC13200.46103551370532847No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGACGT9210.32167705160803595No Hit
ATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCTT8340.29129062002291206RNA PCR Primer, Index 15 (95% over 21bp)
CTTATACACATCTCCGCGCCCACGAGACATAAGTTAATCTCGTATGCCGT6680.23331191148118136No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGATAATCTCGTATGCCGT6650.23226410349548743No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCAGT6480.22632652490988853No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTAAATCTCGTATGCCGT6020.21026013579591493No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATATCGTATGCCGT5910.20641817318170388No Hit
CTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTC5730.2001313252675403No Hit
CTCTAATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC4440.15507558188270137No Hit
TCTTAAACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG4080.14250188605437425No Hit
TCTTAATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC3800.13272234485456424No Hit
CTTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG3760.13132526754030568No Hit
CTTAAACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGACGT3540.12364134231188353No Hit
ATACCCATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCTT3500.12224426499762497RNA PCR Primer, Index 15 (95% over 21bp)
CACTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC3290.11490960909776747No Hit
CCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG3230.11281399312637962No Hit
TTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTC3060.10687641454078069No Hit
CTTAAACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCAGT2990.10443152924082819No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAA455.456968E-1270.077348
ACGAATT150.002213480670.077347
TCTGAAT353.6725396E-970.0650553
TCGAATT150.002215016670.0650544
TGATAGA600.070.05279555
ATTCGGG150.002222708770.00384537
GGTTTGC150.002225790969.9793920
CGTTATT150.002225790969.9793929
TAACCGA252.8405066E-669.9793911
ATAACCG252.8405066E-669.9793910
AGGGGGG59800.064.5963670
CCGTCTA2100.063.4032747
CATAAAC7850.063.293461
TCTTTAT2250.062.2039071
TAAACAC21650.061.898633
CTTAAAC14250.061.3854331
AACACAT22150.061.2912035
CGACTAA352.9454532E-760.06625748
AAACACA24950.060.0224084
TTAAACA15200.058.699822
Produced by FastQC (version 0.11.5)